摘要
目的观察缺血预处理对脑缺血再灌注损伤的保护作用。方法将SD老年大鼠分为2组缺血预处理10min再灌注24h再次缺血24h组(PI)及单纯缺血对照组(CI),采用尼龙线栓法制作大鼠局灶性脑缺血模型,通过免疫组化染色技术及原位末端标记技术(TUNEL)检测大鼠大脑皮质bcl-2蛋白的表达和细胞凋亡情况。结果PI组较CI组缺血皮层bcl-2蛋白表达明显增强(P<0.01)。PI组缺血皮层凋亡细胞较CI组明显减少(P<0.05)。结论缺血预处理使缺血脑组织bcl-2蛋白表达增强,抑制凋亡细胞产生,说明缺血预处理对再次脑缺血有保护作用。
Objective To observe the protective effects of cerebral ischemia preconditioning on cerebral ischemic injury caused by subsequent middle cerebral artery occlusion （MCAO） in SD rats. Methods Male Sprage-Dawley rats were divided into two groups. The first group（PI） ： 10 minutes of ischemic precondition, 24 hours of reperfusion, and then 24 hours of occlusion of the right middle cerebral artery （MCA） ; the second group （CI） ： occlusions of MCAO were performed by inserting nylon suture into the internal carotid artery. Immunohistostaining and TUNEL staining technique were used to examine bcl-2 protein expression and neuronal apoptosis on ischemic cortex. Results In the PI group positive bcl-2 protein expression in the ischemic cortex was more notable than that in the CI group （ P 〈 0. 01 ） ; Cell apoptosis in the ischemic cortex of CI group is much more than that of PI group （ P 〈 0. 05）. Conclusions Ischemic preconditioning increases expression of bcl-2 protein and inhibits cell apoptosis, so it may induce ischemic brain tolerance and has protective effects.
出处
《实用老年医学》
CAS
2005年第5期251-252,共2页
Practical Geriatrics
基金
江苏省预防医学重点课题资助项目(Y200402)
