摘要
目的探讨充血性心力衰竭(CHF)患者外周血单个核细胞产生白介素(IL-6)和肿瘤坏死因子(TNF-α)的变化及其临床意义。方法采用双抗体夹心(ELISA)法测定26例CHF患者治疗前后IL-6、TNF-α水平变化,同时测定10例正常人IL-6、TNF-α水平作为对照。结果CHF患者血浆中IL-6和TNF-α水平明显高于正常对照组(IL-6:920.60±102.58 ng/L和1398.80±143.69ng/L,P<0.05;TNF-α:125.5±40.25 ng/L和278.89±55.36 ng/L,P<0.05)。26例心衰患者治疗后IL-6和TNF-α水平较治疗前显著降低(IL-6:1130.13±138.52 ng/L和1356.63±151.72 ng/L,P<0.01;TNF-α:271.64±58.36 ng/L和209.75±51.48 ng/L,P<0.01)。结论心力衰竭时炎性细胞因子增加,而经药物治疗后,炎性细胞因子降低可能是对CHF有益作用的机制之一。
Objective To evaluate the effect of interleukin-6（IL-6） and tumor necrosis factor-alpha（TNF-α） sereated by peripheral blood mononuclear cells（PBMCs） in congestive heart failure （CHF） before and after treatment . Methods Level of IL-6 and TNF-α were measured by Enzyme-Linked Immuno Sorbent Assay（ELISA） in 26 CHF patients before and after treatment and in 10 healthy donors. Results Compared with control, IL-6 and TNF-α level in patients with CHF was significantly higher（IL-6： 920.60 ± 102.58ng/L, versus 1398.80 ± 143.69ng/L, P 〈 0.05 ; TNF-α： 125.5 ± 40.25 ng/L, versus 278.89 ± 55.36 ng/L, P 〈 0.05）. After treatment, IL-6 and TNF-α level in 26 patients with CHF decreased obviously than before （ IL-6：1930.13 ± 13.52ng/L, versus 1356.63 ± 151.72 ng/L, P 〈 0.01 ; TNF-α： 271.54± 58.36ng/L,versus 209.75 4- 51.48ng/L, P 〈 0.01）. Conclusions Inflammatory cytokines in CHF patients rised,while they decreased after treatment. Reducing inflammatory eytokines may be one of the beneficial effects on CHF.
出处
《中国血液流变学杂志》
CAS
2005年第3期474-475,共2页
Chinese Journal of Hemorheology
