摘要
目的:建立用高效液相色谱法(HPLC法)测定人血浆样品中阿德福韦浓度的定量方法。方法:应用Agilent 1100高效液相色谱仪,以Eclipse XDB C18柱为色谱柱(150 mm×4.6 mm,5μm),流动相为0.2%H3PO4-甲醇(87:13),检测波长为260 nm,流速为1.0 mL/min。结果:阿德福韦浓度在20-2 000 ng/mL范围内与峰面积线性关系良好(r=0.999 3)。结论:HPLC法简便、快捷、灵敏、准确,适用于阿德福韦药代动力学研究.
Objective： To establish HPLC method for assaying adefovir in human plasma. Methods： Adefovir in the samples was quantitatively analyzed by HPLC with the external standard. HPLC conditions for adefovir were as follows： Agilent 1100 HPLC instrument was used with C18 column（150 mm × 4. 6 mm,5 μm）. The mobile phase consisted of 0. 2% H3PO4 -CH3OH in plasma with the flow rate 1.0 mL/min. They were determined at UV wavelength of 260 nm. Re, suits： The nicer linear range was 20-2 000 ng/mL of adefovir concentration（r=0.999 3）. Conclusion： HPLC is simple, rapid, accurate, and suitable for application to study on pharmacokinetics of adefovir.
出处
《中国药业》
CAS
2005年第10期68-69,共2页
China Pharmaceuticals
