摘要
目的：观察硫酸镁对大鼠肺缺血-再灌注损伤的影响。 方法：实验于2003-05／2004-05河南科技大学医学院机能学实验室完成。将Wistar大鼠随机分为正常组、实验组(缺血-再灌注损伤组)及治疗组(硫酸镁组)，每组10只。戊巴比妥钠腹腔注射麻醉固定后，颈部气管切开插管，胸骨正中切开，尾静脉注射1mg／kg肝素90min后，实验组和治疗组在吸气末用无创伤血管夹阻断左肺门30min，放开后再灌注60min，制备大鼠原位肺缺血-再灌注损伤模型，治疗组于左肺门阻断前5min及再灌注前5min分别由尾静脉注入50g／L硫酸镁溶液20mg／kg，正常组手术方法同实验组和治疗组，但不阻断左肺门。各组在实验前后及灌注前后分别测定肺动脉氧分压及血氧饱和度，注射硫酸镁后测定血浆及肺匀浆超氧化物歧化酶及丙二醛含量，并计算肺组织湿于比值，观察肺组织病理变化。 结果：治疗组与实验组比较，肺湿干重比显著降低(t=5．548，P<0．01)，肺动脉氧分压及氧饱和度显著升高(t=3．132，7．150，P<0．01)；血清及肺匀浆超氧化物歧化酶活性显著增高(t=3．81，3．791，P<0．01)，丙二醛含量降低(t=13．35，3．32，P<0．01)。与正常组比较肺湿干重比显著升高(t=8．632，P<0．01)，动脉氧分压及氧饱和度显著降低(t=18．044，10．715，P<0．01)，血清及肺匀浆超氧化物歧化酶活性显著降低(t=4．93，11．33，P<0．01)，丙二醛含量增高(t=15．61，3．13，P<0．01)。实验组肺组织病理显示有大量炎性细胞浸润，有明显点块状出血坏死灶，间质明显水肿，治疗组仅见少量点状出血点及轻度炎性细胞浸润。 结论：镁制剂可通过清除氧自由基、抗氧化、减少脂质过氧化及激活腺苷酸环化酶，对大鼠肺缺血-再灌注损伤起保护作用。
AIM： To observe the effect of magnesium sulfate on lung ischemia-reperfusion injury in rats. METHODS： The experiment was completed in the Enginery Laboratory of Medical College, Henan University of Science and Technology from May 2003 to May 2004. Thirty Wistar rats were randomly divided into normal group （n=10）, experimental group （ischemia-reperfusion injury group, n=10） and treatment group （magnesium sulfate group, n=10）. After the intraperi- toneal injection of pentobarbitone natricum, the rats were anaesthesia fixed, it was cut open in the neck windpipe and the tube was put in it, and then the breastbone was cut open in the middle. After the caudal venous injection of 1 mg/ kg heparinum for 90 minutes, the rat models of lung isehemia-reperfusion injury in the experimental group and treatment group were established by ligating the hilum of left lung for 30 minutes followed by reperfusion for 60 minutes. At 5 minutes hefore the hilum of left lung was ligated and 5 minutes before reperfusion, rats in the treatment group were given caudal venous injection of 50 g/L magnesium sulfate solution （20 mg/kg） respectively. The operative methods in the normal group were the same as those in the experimental group and treatment group, but the hilum of left lung was not ligated. Before and after the experiment and reperfusion, the pulmonary artery pressure of oxygen and oxygen saturation were determined. The activity of superoxide dismutase （SOD） and content of malondialdehyde （MDA） in plasma and pulmonary homogenate after in- jection of magnesium sulfate, and the lung wet/dry ratio was calculated, and the pathological changes of lung tissue were observed. RESULTS： Compared the treatment group with the experimental group, the lung wet/dry ratio was significantly decreased （t=5.548, P 〈 0.01）, pulmonary artery pressure of oxygen and oxygen saturation were significantly increased （t=3.132, 7.150, P 〈 0.01）, the SOD activities in plasma and pulmonary homogenate were markedly increase
出处
《中国临床康复》
CSCD
北大核心
2005年第35期87-89,共3页
Chinese Journal of Clinical Rehabilitation
