摘要
在吉林省内采集甜椒炭疽病病样,经分离、纯化、观察,确定Colletotrichum nigrum Ell.et.Halst(黑色炭疽病)为主要病原菌,并以此作为接种源,对接种液浓度、接种时期、接种方法、接种后温度、湿度及调查时间、调查标准等进行了一系列研究.最后确定用3×106个/mL的孢子悬浮液,在成熟红果期采用离体果针刺法接种,接种后温度(27±1)℃,遮光保湿3 d,保持相对湿度(RH)为95%～100%,7 d后调查病情指数,进行品种抗病性评价.用此抗性鉴定方法对21份甜椒材料进行了筛选鉴定,5份材料表现抗病,10份表现耐病,6份表现感病.
The anthracnose samples of sweet pepper collected in Jilin province were isolated, purified and investigated. It was found that Collototrichum nigrum Ell. et. Halst was the main pathogen in Jinlin province. This pathogen was inoculated on harvested red fruit by injected inoculation. The research showed that the best conditions of inoculation were： the temperature was 27 （ ±1 ） ℃, RH was kept between 95% and 100% for 72 h in dark, and the concentration was 3 × 10^6 spore/mL. After 7 days, the disease indexes were investigated and the resistance was evaluated. At the same time, 21 varieties of sweet pepper were screened and identified with this identifying method. The result showed that 5 are of resistance, 10 are of tolerance, and 6 are of sensitivity. This method is helpful to resistance breeding.
出处
《吉林农业大学学报》
CAS
CSCD
北大核心
2005年第5期507-510,共4页
Journal of Jilin Agricultural University
基金
国家"863"计划资助项目(2202AA7013-8)
关键词
甜椒
炭疽病
抗性鉴定
抗源筛选
sweet pepper
anthracnose
identification
screening
