摘要
目的:研究克林沙星与小牛胸腺DNA之间的相互作用。方法:使用荧光光谱法,根据Stern-Volmer方程及Scatchard方程进行数据处理。采用离子强度的影响、紫外光谱的变化、单双链DNA作用的区别及I-猝灭等实验研究了克林沙星与小牛胸腺DNA间的相互作用模式。结果:在pH值7.0的磷酸盐缓冲液(PBS)中,克林沙星的荧光激发及发射峰分别位于273 nm和427 nm处,小牛胸腺DNA对克林沙星的荧光存在着强烈的猝灭作用,猝灭常数Kq为4.03×104mol-1.L,结合位点数为2.84。结论:克林沙星与小牛胸腺DNA间是一种沟槽作用模式。
Objective： To investigate the interactions between clinafloxacin and calf thymus DNA Methods： The interaction mode was studied by using fluorescence spectrometry. The experimental data were plotted by Stern-Volmer equation and Scatchard equation. Based on ion strength effects, UV spectra, the differences of single stranded DNA from double stranded DNA bound to clinafloxacin and I-quenching effects, the binding interaction mode between clinafloxacin and calf thymus DNA was studied. Results： At pH 7.0 PBS, the excitation and emission wavelength of clinafloxacin were 273 nm and 427 nm, respectively. The addition of calf thymus DNA to clinafloxacin solution resulted in a strong fluorescence quenching with a quenching constant Kq = 4.03 × 10^4mol^-1· L and the binding site size in DNA base pairs n = 2.84. Conclusion： Clinafloxacin interacts with calf thymus DNA in groove bindings.
出处
《中国新药杂志》
CAS
CSCD
北大核心
2005年第10期1190-1193,共4页
Chinese Journal of New Drugs
基金
中国药科大学青年教师科技基金(D0319)
