摘要
目的观察皮肤鳞状细胞癌(鳞癌)和基底细胞癌(基癌)中细胞凋亡及其与c-fos、BNIP1表达的关系。方法应用原位末端标记和原位杂交技术检测48例皮肤鳞癌和41例基癌标本中细胞凋亡及C-fos、BNIP1 mRNA表达。结果鳞癌中凋亡指数(AI)、c-fos mRNA表达明显高于基癌(P＜0．01),但BNIP1 mRNA表达在二者间的差异无显著性(P＞0．05)。AI在高分化鳞癌中明显增多(P＜0．05),但C-fos表达以低分化鳞癌更为明显(P＜0．05)。鳞癌中AI与c-fos、BNIP1表达呈显著正相关(P＜0．05)。结论鱗癌中细胞凋亡增多与c-fos表达上调相关,BNIP1在鳞癌和基癌中可能起促凋亡作用。
Objective To study the relationship between cell apoptosis and mRNA expression of c-fos and BNIP1 in cutaneous squamous cell carcinoma （SCC） and basal cell carcinoma （BCC）. Methods Cell apoptosis and mRNA expression of c-fos and BNIP1 in 48 SCCs and 41 BCCs were determined by TUNEL and in situ hybridization, respectively. Results Apoptosis index （AI） and e-los mRNA expression in SCCs were higher than those in BCCs （P 〈 0.01 ）, but BNIP1 mRNA expression did not differ between them （P 〉 0.05）. AI was significantly higher in well-differentiated SCCs than in poorly differentiated SCCs （P 〈 0,05）, which was contrary to the expression of c-fos. Significant positive correlation was found between AI and expression of e-fos and BNIP1 （P 〈 0.05） in SCCs. Conclusions The increase of AI in SCCs is related to e- fos overexpression. BNIP1 is likely to induce the apoptosis in SCC and BCC.
出处
《中华皮肤科杂志》
CAS
CSCD
北大核心
2005年第10期631-633,共3页
Chinese Journal of Dermatology
基金
湛江市科技攻关项目(湛科[2003]69-7)
