摘要
目的探讨Ras反义寡核苷酸(ASODN)对胰腺癌Pc-2细胞多药耐药的影响。方法应用RasASODN抑制Pc-2细胞中Ras和P-糖蛋白(P-glyoprotein,P-gp)表达,采用MTT法测定Pc-2细胞化疗敏感性,荧光定量RT-PCR法检测细胞内MDR-1基因水平,流式细胞仪检测细胞内阿霉素(ADR)浓度的改变。结果RasASODN能明显抑制胰腺癌Pc-2细胞中Ras和P-gp蛋白的表达(P<0.05),提高Pc-2细胞的化疗敏感性(P<0.05),降低Pc-2细胞中MDR-1基因水平(P<0.05),增加Pc-2细胞中的ADR摄入量(P<0.05)。结论RasASODN可能通过调控MDR-1基因水平,增加多药耐药胰腺癌Pc-2细胞对化疗药物的敏感性。
Objective To investigate the role of Ras antisense oligodexynucleotide （ASODN） in multidrug resistance （MDR） of pancreatic carcinoma Pc-2 cells. Methods Ras and P-gp expression was suppressed by Ras ASODN. Sensitivity of Pc-2 cells to chemotherapy was determined by the MTT assay. MDR-1 mRNA level was detected by fluorogenic probe quantitative reverse transcription polymerase chain （RT-PCR） method. Flow cytometry （FCM） was used to detect the accumulative concentration of adriamycin （ADR） in the cells. Results Ras ASODN significantly inhibited the Ras and P-gp expression （P〈0.05）, increased the sensitivity of Pc-2 cells to chemotherapeutic agents （P〈0.05）, decreased MDR-1 gene level in Pc-2 cells （P〈0.05）, and increased the intracellular intake of ADR in Pc-2 cells （P〈0.05）. Conclusion Ras ASODN may enhance the sensitivity of multidrug-resistant pancreatic cancer Pc-2 cells to chemotherapeutic agents by regulating MDR-1 gene level.
出处
《中国医学科学院学报》
CAS
CSCD
北大核心
2005年第5期633-636,共4页
Acta Academiae Medicinae Sinicae
关键词
胰腺癌
多药耐药
RAS
pancreatic carcinoma
multidrug resistance
Ras
