摘要
按一定比例混合脱脂豆粕和反渗透水,混合液在pH 8.0及室温下连续搅拌40min,再经离心去渣得到大豆蛋白提取液.将提取液在适当的条件下酶解-超滤,截留液经冷冻干燥得到大豆改性蛋白.所制得产物的蛋白质含量(质量分数)从52.6%提高到78%以上,回收率大于80%.SDS-PAGE凝胶电泳结果显示,经Alcalase 2.4L改性的大豆蛋白主要含glycinin的碱性亚基,经fungal protease concentrate改性的大豆蛋白主要含glycinin的酸性亚基和碱性亚基.
The soy protein （SP） supernatant, obtained by the centrifugation of defatted soy flour suspension in preweighted reverse osmosis water at pH 8.0 after continuous agitation for 40 min, was selectively hydrolyzed by protease in certain conditions, and was then subjected to ultrafiltration for fractionation. The retentate was lyophilized to obtain modified soy protein with the protein content （mass fraction, below the same） increasing from 52.6% to more than 78%, and the yield more than 80%. The results of SDS-PAGE show that the soy protein modified by Alcalase 2.4L mainly contains the basic subunits of glycinin, and that modified by fungal protease concentrate mainly contains the acid and basic subunits of glycinin.
出处
《华南理工大学学报（自然科学版）》
EI
CAS
CSCD
北大核心
2005年第10期78-83,共6页
Journal of South China University of Technology(Natural Science Edition)
基金
"十五"国家科技攻关项目(2001BA501A02)广东省"十五"科技攻关项目(A20301)~~
关键词
大豆蛋白
改性
超滤
选择性酶解
soy protein
modification
ultrafiltration
selective enzymolysis
