摘要
目的:建立滇姜花Hedychium yunnanense的二萜类成分姜花酮(hedychenone)的含量分析方法.方法:C18柱,流动相乙腈-水(9:1),柱温25℃,流速1.0 mL·min-1,检测波长235 nm.结果:hedychenone的线性范围5.92～29.6μg·mL-1(r=0.999 9).平均加样回收率为98.9%.精密度RSD＜2%(n=5).结论:本方法简单、有效、可行,可用于滇姜花的质量评价.
Objective： To establish an HPLC method for determination of hedychenone in Hedychium yunnanense. Method： C18 Chromatographic column was used, acetonitrile-water （9：1 ） as mobile phase, at flow rate of 1.0 mL·min^-1. The wavelength for detection was 235 nm. Result： The linear range of hedychenone was 5.92-29.6μg· mL^-1（ r = 0.999 9）. The average recovery was 99.0 %, RSD of precision was less than 2%. Conclusion： The method is simple, effective and feasible, and can be used to evaluate the quality of the herb.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2005年第20期1593-1595,共3页
China Journal of Chinese Materia Medica
关键词
滇姜花
姜花酮
HPLC
Hedychium yunnanense
hedychenone
HPLC
