期刊文献+

转铁蛋白载体携带阿霉素的分子间结合作用

Interaction between Transferrin and Adriamycin
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摘要 利用荧光光谱法和吸收光谱法研究阿霉素与转铁蛋白间的结合作用,确定了阿霉素对转铁蛋白的荧光猝灭过程的猝灭机理,测定了不同浓度下该结合反应的结合常数K=(0.98~89.1)×105,结合位点数n=1.09~1.37,并依据能量转移理论确定了药物和蛋白间的结合距离r=1.94 nm. To understand the interaction between adriamycin (ADR) and transferrin (Tf) solution, the binding reaction between ADR and Tf was investigated by fluorescence and ultraviolet visible absorption ( UVVis) spectroscopy methods. The mechanism of the fluorescence quenching of Tf induced by ADR was determined. The relation between concentration of ADR and Fo/F was linear. It showed that the quenching of Tf induced by ADR was either dynamic or static quenching. The ultraviolet absorption of Tf decreasing in the presence of ADR showed that a nonluminous compound was formed by the interaction of Tf and ADR. So it was confirmed that the mechanism of the fluorescence quenching of Tf induced by ADR was static quenching. Furthermore, we use Stern-Volmer equation to approve it. The equilibrium constants K were measured equaling to (0.98×89.1) × 10^5 while the numbers of binding sites n were 1.09 × 1.37 at different concentration by means of fluorescence quenching method. The binding distance r = 1.94 nm in the interaction of 6.9 × 10^-5 mol/L ADR and 5.6 × 10^-6 mol/L Tf was also obtained according to Fǒrster theory of non-radiation energy transfer. A kind of binary compound of ADR and Tf for the preparation of oriented drug carried by Tf. was formed, providing the instructional meaning
出处 《发光学报》 EI CAS CSCD 北大核心 2005年第5期669-673,共5页 Chinese Journal of Luminescence
基金 广东省自然科学基金(021190) 广州市科技计划项目基金(2003Z3-D2401)资助项目
关键词 阿霉素 转铁蛋白 荧光光谱法 吸收光谱 adriamycin (ADR) transferrin (Tf) fluorescence spectroscopy absorption spectroscopy
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