摘要
目的探讨surv iv in在胶质母细胞瘤中的表达及与其凋亡、增殖和微血管密度(M VD)的相关性。方法应用免疫组织化学(SP)法检测42例份胶质母细胞瘤石蜡切片中surv iv in、K i-67和第8因子相关抗原(FⅧRA g)的表达情况,并用末端转移标记法测定凋亡指数(A I)。结果surv iv in在胶质母细胞瘤中的表达阳性率为81.0%,而正常对照组中无1例阳性表达;surv iv in的表达与K i67及M VD呈正相关,而与A I负相关。结论surv iv in在胶质母细胞瘤的发生和发展中可能通过调控细胞的凋亡和增殖起着重要作用,并参与肿瘤血管的生成。可望成为诊断治疗的新靶点。
Objective： To investigate the expression of survivin in glioblastomas and its relation with apoptosis, proliferation and microvessel density（MVD）. Methods： The expression of survivin and Ki-67, factor Ⅶ-related antigen（FⅦRAg） was detected by immunohistochmical （SP） method in 42 glioblastomas and 10 cases normal brain tissue. Apoptosis index（AI）were detected by TdT-Meadiated dUTP nick end labeling. Results： No survivin expression was detected in the tissue samples from normal brain, while it was detected in 34 of 42 tissue samples from glioblastomas （81.0%）. The expression of survivin protein was positively correlated with Ki-67 and MVD, but inversely correlated with AI （P 〈0.05）. Conclusions： Overexpression of survivin in the glioblastomas can inhibit apoptosis and promote proliferation, and play an important role in tumor angiogenesis. Survivin may be identified as a potential targets for diagnosis and treatment.
出处
《山东医药》
CAS
北大核心
2005年第30期7-8,共2页
Shandong Medical Journal
基金
留学回国人员科研启动基金[教外司留(2002)247号]
