摘要
用MnO2将佛波醇-12-肉豆蔻酯-13-乙酯氧化,然后利用新制的NaB3H4氚化还原合成[20-3H]佛波醇-12-肉豆蔻酯-13-乙酯。所得产物经HPLC纯化后放化纯度>98%,其比活度达0.654 TBq/g。纯化产物在-20℃放置90天仍保持稳定。本法简单易行,标记过程中也不产生异构体。
[-20-^3H]phorbol-12-myristate-13-acetate is systhesised by reduction of NaB^3H4 after using MnO2 to oxidize phorbol-12-myristate-13-acetate. The radiochemical purity of the product purified by HPLC is more than 98%, and its specific activity is 0. 654 TBq/g. The purified product remains stable at -20 ℃ during 90 days. This is a simple and efficient way, and no isomer is produced in the process.
出处
《同位素》
CAS
北大核心
2005年第3期153-156,共4页
Journal of Isotopes
基金
中国原子能科学研究院院长基金资助项目(ZJ200314)
