摘要
依据微生物在自然生境中协作生长的基本特性,提出一种对传统纯培养技术的改进思路及方法:设计一种有孔培养皿,皿内覆盖有不允许细菌通过、但营养物质可以自由流动的微孔滤膜。培养时将该培养皿放入被分离微生物所需生境中,可以克服传统纯培养难以提供外源活性物质的缺陷,一定程度上弥补了混合培养法和传统纯培养法的弱点,从而达到增强部分微生物可培养性、甚至培养出未培养微生物的目的。
Based on the ecological features of mutual benefits of different microbes in natural environment, near-natural pure culture technique which was an improvement of conventional pure culture method had been put forward. The key point of this new method was to make hole petri dishes covered with millipore membranes. When these covered dishes being used to culture microorganisms, it could provide incubated microbes active materials from native environment, maintain free communicating with outside microbes, and improve their recovery and culturability of some bacteria. To some degree , this technique enhanced their strengths and overcome their weaknesses of mixed culture method and conventional pure culture method so as to increase microbial culturability and even possibly obtain some nonculturable microorganisms.
出处
《微生物学报》
CAS
CSCD
北大核心
2005年第5期802-804,共3页
Acta Microbiologica Sinica
基金
国家自然科学基金(50378095)
重庆大学骨干教师基金(716411045)~~
关键词
近自然纯培养法
可培养性
未培养微生物
Near-natural pure culture technique, Culturability, Nonculturable microorganism
