摘要
对虾白斑综合症其病原是对虾白斑综合症病毒(Whitespotsyndromevirus,简称WSSV)。VP19是WSSV的一个囊膜蛋白,HyNPV(HybridofAcNPVandBmNPV,简称HyNPV)是BmNPV和AcNPV通过基因重组后得到的一个具有BmNPV和AcNPV双重优点的新型杂交病毒,在克隆了VP19基因的基础上,成功构建了重组转移载体pBlueBicHisCvp19和重组杆状病毒HyNPVVP19。用重组病毒注射接种5龄起蚕,经SDSPAGE和Westernblotting分析,结果表明,WSSVVP19基因在家蚕体内得到了表达,特异性条带大小与预计的基本一致,约为21kD。
Prawn white spot syndrome is caused by the pathogen prawn white spot syndrome virus （WSSV）. VP19 is a vesicle membrane protein of WSSV. HyNPV （Hybrid of AcNPV and BmNPV） constructed by the recombination of BmNPV and AcNPV is a new hybrid virus having both of their advantages. The recombinant transfer vector pBlueBicHisC-vpl9 and recombinant baculovirus HyNPV-VP19 were constructed on the basis of the successful cloning of VP19. Newly-molted silkworms Bombyx mori of fifth instar were inoculated by the recombinant virus. SDS-PAGE and Western blotting analysis showed a specific band, about 21kD, which was consistent with the expectation suggesting that the WSSV-VP19 gene was successfully expressed in silkworm bodies.
出处
《生物工程学报》
CAS
CSCD
北大核心
2005年第5期837-839,共3页
Chinese Journal of Biotechnology
基金
江苏省高校自然科学研究计划项目(No.03KJB240125)
苏州大学博士启动基金项目。~~
