摘要
目的探讨肿瘤坏死因子相关诱导凋亡配体的4种受体DR4、DR5、DcR1、DcR2在肝细胞癌肝组织中的表达状况。方法应用半定量逆转录聚合酶链反应(RTPCR)方法检测40例人肝细胞癌组织、相应癌旁肝组织、23例正常人肝组织中DR4、DR5、DcR1、DcR2的mRNA表达率及表达水平。结果(1)40例肝癌组织、癌旁组织、23例正常肝组织DR4、DR5的mRNA的表达水平差异无统计学意义(P>0.05);(2)40例癌旁组织、23例正常肝组织DcR1、DcR2表达水平明显高于40例肝癌组织(P<0.05);(3)DR4和DR5mRNA在肝细胞癌组织中表达水平与患者的年龄,肿瘤大小,有无包膜,分级程度,AFP水平,HBsAg,有无肝硬化有关系。结论肝癌组织中存在TRAIL受体的表达,与其在正常肝组织中的表达差异有统计学意义(P<0.05);DR4、DR5表达水平与肝癌的病理状况有关系。
Objective To explore the mechanism and clinical significance of TRAIL-induced apoptosis in hepatocellular carcinoma （HCC） by detecting the expression of DR4, DR5, DcR1 and DcR2 mRNA, the main recepters of the TRAIL, in liver tissue of patients with HCC. Methods DR4, DR5, DcR1 and DcR2 mRNA expression and its levels were assessed in 23 cases of normal liver tissue, 40 cases of para-liver cancer tissuse and 40 cases of HCC tissues with semi-quantitative reverse transcription polymerase chain reation （RT-PCR） method. Results （1） There was no different expression levels of DR4 and DR5mRNA among the normal liver tissue, para-liver cancer tissuse and HCC tissue （P 〉 0.05）;（2） The positive expression level of DcR1 and DcR2 mRNA in the normal liver tissue and para-liver cancer tissuse was significant1：, higher than in HCC tissue （P 〈 0.05）;（3） Statistical correlation hmong the expression changes of DR4 or DR5 mRNA and patient＇ s age, size and differentiation of the tumor, plasma AFP levels and HBsAg was existed. Conclusion TRAIL receptors expression was prevalent in HCC, which was different from that in normal liver tissue;The expression of DR4 or DR5 was closely correlated with HCC pathological status.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2005年第10期1214-1216,共3页
Chinese Journal of Experimental Surgery
