摘要
以杂交鹅掌楸的叶片、叶柄、无菌苗的茎段及其芽基部切段为外植体,进行愈伤组织途径的器官发生及不定芽途径的直接器官发生培养。结果表明,多种外植体在诱导培养基上均能脱分化产生愈伤组织,其中无菌苗芽基部具有最高的愈伤组织诱导率。愈伤组织在MS+6-BA 2.0 mg.L-1+NAA 0.5 mg.L-1和MS+6-BA 4.0 mg.L-1+NAA 0.5 mg.L-1的分化培养基上能分化出不定芽。部分外植体在MS+6-BA 4.0 mg.L-1+NAA 0.5 mg.L-1的分化培养基上直接分化产生不定芽。器官发生途径再生体系的建立为抗逆基因工程等工作奠定了基础。
The organogenesis systems of Liriodendron hybrids were established by using the leaves, petioles, stem fragments and basal shoot fragments as explants. The results indicated that the callus could be induced from these explants on the inductive media, and the basal shoot fragment had the highest callus-inducing rate. The calli differentiated into shoot bud on the differentiation media MS ＋ 6-BA 2.0 mg · L^-1 ＋ NAA 0.5 mg· L^-1 and MS ＋ 6-BA 4.0 mg · L^-1 ＋ NAA 0.5 mg · L^-1, and some explants directly differentiated into shoot on the medium MS ＋ 6-BA 4.0 mg· L^-1 ＋ NAA 0.5 mg - L^-1. The organogensis systems offered base for the molecular breeding.
出处
《林业科学研究》
CSCD
北大核心
2005年第5期546-550,共5页
Forest Research
基金
浙江省科技厅重点项目(2003C22011)
中国林科院亚热带林业研究所青年科技发展基金项目(2004)的部分研究内容
