摘要
目的:观察前列地尔对马兜铃酸肾病(AAN)纤维化的防治作用。方法:Wistar大鼠25只,随机分3组,对照组(n=5)予灌服饮用水10mL·kg-1·d-1,模型组及治疗组(均n=10)均予关木通水煎剂20g·kg-1·d-1,共计12wk。治疗组8wk后同时予尾静脉注射前列地尔4μg·kg-1·d-1,共2wk。12wk时观察各组大鼠尿素氮(BUN)和肌酐(Cr)、肾组织转化生长因子β1(TGF-β1)、结缔组织生长因子(CTGF)和纤连蛋白(FN)的变化。结果:模型组大鼠BUN,Cr较对照组和治疗组明显升高(P<0.01);模型组中TGF-β1,CTGF和FN光密度明显高于对照组和治疗组(P<0.05);治疗组大鼠肾脏病理显示肾损害较模型组明显减轻。结论:前列地尔能使AAN大鼠肾组织TGF-β1,CTGF和FN表达下降,减轻马兜铃酸肾病纤维化程度。
AIM：To evaluate the mechanism of the effect of alprostadil on preventing the fibrosis of aristolochic acid nephropathy （AAN）. METHODS ： Twenty-five Wistar rats were divided randomly into 3 groups. The rats in the control group （ n = 5 ） were fed with water （10 mL · kg^-1· d^-1）. The rats in the model group （n = 10） and the therapy group （n = 10） were fed with Aristolochia manshuriensis （20 g · kg^-1· d^-1 ） for 12 wk respectively and furthermore the rats in the therapy group were simultaneously given alprostadil （4 μg · kg^-1· d^-1） at the 8th week these afterward via the caudal vein. The serum levels of BUN, Cr and TGF-β1, CTGF,FN in nephridial tissue were analyzed after 12 wk respectively together with renal histology analysis. RESULTS： The hemoglobin and weight in model group was significantly lower and urinary protein excretion was higher than that of control group. BUN and Cr in model group were significantly higher than those in the other two groups （P 〈0.01 ）. Renal histologic damage in therapy group was obviously less serious than that of the model group. The optical densities of TGF-β1, CTGF and FN with immunohistochemical in model group changes were significantly higher than those of the other two groups （P 〈 0.05 ）. CONCLUSION： The results indicate that alprostadil can prevent and cure the fibrosis of AAN by lowing the expression of TGF-β1, CTGF and FN in renal tissue of AAN rat.
出处
《中国新药与临床杂志》
CAS
CSCD
北大核心
2005年第10期773-777,共5页
Chinese Journal of New Drugs and Clinical Remedies
关键词
前列地尔
肾病
纤维化
大鼠
关木通
alprostadil
nephrosis
fibrosis
rats
Aristolochia manshuriensis
