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核糖体失活蛋白体内外抗病毒的实验研究 被引量:6

Experimental Study of RIP to Resist Virus in vitro
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摘要 核糖体失活蛋白是一类广泛存在于高等植物细胞内的能抑制核糖体功能的植物蛋白。建立了从苦瓜籽中提纯核糖体失活蛋白的方法,蛋白质电泳结果显示提取液中含有一定量的核糖体失活蛋白。在鸡胚成纤维细胞和鸡胚上进行核糖体灭活蛋白体内外抗马立克病病毒、新城疫病毒和鸡痘病毒试验,结果均表明核糖体灭活蛋白对3种病毒有一定的抑制作用。 Ribosome inactivating protein (RIP) is a kind of toxin plant protein in which extensively lives in the body of higher plants and controls ribosome's function. Beside it can control proteins combination, it has lots of biological reactivity as resisting giving birth and tumor and controlling HIV. At first, RIP is isolated from seeds of bitter melon. The result of SDS-PAGE indicates that there are lots of RIP in the abstraction liquid. Then we study the antiviral action of RIP through the ceil of CEF and SPF chicken embryos. The results show that RIP can resist NDVF48E8, MDVCV1988 and FPV-SD4 to some extent.
出处 《微生物学通报》 CAS CSCD 北大核心 2005年第5期107-113,共7页 Microbiology China
关键词 核糖体失活蛋白 细胞病变 MTT 血凝 RIP, CPE, MTT, HA
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