摘要
目的:探讨IL-1B基因多态性影响幽门螺旋杆菌(helicobacter pylori,H.pylori或Hp)感染后胃黏膜IL-1β的表达。方法:采用PCR-限制性长度片段多态性(restriction frag-ment length polymorphism,RFLP)分析法检测胃癌低发区117名普通人群IL-1基因多态性。同时比较不同基因型普通人胃黏膜H.pylori阳性和阴性者的IL-1β表达的差异。结果:在胃窦组织中,H.pylori阴性个体的IL-1βmRNA的表达量极少,而H.pylori阳性个体有明显的表达;但在IL-1B-511T/T、C/C和C/T基因型之间差异无统计学意义,P=0·066。在胃体组织中,H.pylori阴性者IL-1βmRNA无明显表达;而在H.pylori阳性者中,IL-1βmRNA表达显著增加(0·44±0·11和0·76±0·14,t=3·2,P=0·02)。而且H.pylori阳性IL-1B-511T/T基因型个体IL-1βmRNA表达比C/T和C/C基因型明显增加(分别为0·86±0·15、0·70±0·16和0·67±0·16,q=2·4和2·7,P=0·043和0·036)。结论:IL-1B基因多态性(-511位点)可促进H.pylori感染后胃体黏膜IL-1βmRNA的表达。肿瘤防治杂志,2005,12(19)
OBJECTIVE： To investigate the effect of interleukin 1B （IL-1B） gene polymorphism on the expression of IL-1β of gastric mucosa with helicobacter pylori （ H. pylori） infection. METHODS： Genomic DNA was extracted from peripheral blood of 117 subjects, including 65 males and 52 females from gastric cancer bigher prevalence region. IL-1 polymorphisms were analyzed by PCR-RFLP. The expression level of IL-1β in different genotypes or H. pylori status was analyzed. RESULTS： IL-1β mRNA expression in subjects without H. pylori infection was rare in antrum tissue, but obvious in subjects with H. pylori infection. There were no marked difference between IL-1B-511T/T genotype and C/C,C/T. In corpus mucosa, obvious IL-1β mRNA expression of all subjects without H. pylori infection was not detected. In subjects infected with H. pylori, however, the expression of IL-1β mRNA increased significantly （0.44±0.11 vs 0.76±0.14, t=3.2, P=0.02）. Subjects with H. pylori infection and IL-1B-511T/ T had higher IL-1β mRNA, compared with C/T and C/C （0. 86±0. 15 vs 0. 70±0. 16 and 0.67±0. 16, q=2.4 and 2.7, P=0.043 and 0.036）. CONCLUSION： Interleukin-1B gene polymorphism （- 511 locus） up-regulates the expression of IL-1β mRNA of corpus in subjects with H. pylori infection.
出处
《肿瘤防治杂志》
2005年第19期1449-1452,共4页
China Journal of Cancer Prevention and Treatment
