摘要
以Thatcher和23个以Thatcher为遗传背景的小麦抗叶锈病近等基因系及TcLr19与Thatcher杂交F2代植株为材料,利用AFLP技术开展了小麦抗叶锈病基因Lr19的分子标记研究。共获得7个与小麦抗叶锈病基因连锁的分子标记:P-AGT/M-GAG289bp(3.3cM)、P-ACA/M-GGT102bp(4.1cM)、P-ACA/M-GGT106bp(4.1cM)、P-AAC/M-CAG123bp(4.9cM)、P-AAC/M-GGT203bp(5.0cM)、P-ACA/M-GGT290bp(5.7cM)和P-ATC/M-GAG293bp(9.6cM)。这些特异性片段经回收、克隆、测序得出了特异带的序列。该研究可促进遗传图谱、物理图谱的构建和小麦抗叶锈病基因Lr19的克隆。
AFLP analysis was carded out on Thatcher, twenty three near- isogenic lines and F2 generation of Tclr19 × Thatcher and developed molecular markers for gene Lr19 resistance to wheat leaf rust. Seven markers linked to Lr19 resistance traits were obtained, which were P-AGT/M-GAG289bp （3.3cM）, P-ACA/M-GGT102bp （4.1cM）, P-ACA/M-GGT106bp （4.1cM）, P-AAC/M-CAG123bp （4.9cM）, P-AAC/M-GGT20abp （5.0cM）, P-ACA/M-GGT290bp （5.7cM）, and P-ATC/M-GAG293bp （9.6cM）. All of these specific fragments were isolated from the polyacrylamide gels, re-amplified, cloned and sequenced. The research may facilitate genetic mapping, physical mapping, and eventual cloning of Lr19 gene.
出处
《中国农业科学》
CAS
CSCD
北大核心
2005年第9期1796-1800,共5页
Scientia Agricultura Sinica
基金
国家自然科学基金项目(30170602)
