摘要
背景:目前在探讨骨缺损的物理和药物方法治疗的效果、检验手术方式的有效性、以及评价骨替代材料在骨缺损修复中所起的作用时,尚缺少一种客观的、标准的动物模型。目的:为骨不连的实验研究建立一种客观的动物实验模型。设计:制作兔骨不连模型的验证性实验。单位:深圳市人民医院骨科。材料:取普通级纯种新西兰大白兔20只,体质量(2.5±0.5)kg,6～8月龄,雌雄不限。方法:实验于1999-05/08在深圳市人民医院动物实验中心完成。选取纯种新西兰大白兔20只,在前臂桡骨中段截除1.5cm骨段(包括骨膜),骨断端用骨蜡封闭髓腔,10周后经大体标本、病理组织学及X射线检查确定骨不连形成情况。主要观察指标:兔前臂桡骨骨缺损模型大体观察及病理组织学和放射学检查结果。结果:纳入白兔20只(40侧桡骨)均进入结果分析。①兔骨缺损区大体观察:10周后骨缺损区可见纤维瘢痕组织填充,无骨性连接,骨蜡未吸收,骨端硬化,髓腔闭塞,两侧断端有少量骨痂形成,骨缺损范围为0.8～1.2cm。②兔骨缺损区病理组织学检查:光镜下骨断端髓腔封闭,有软骨细胞及骨细胞,细胞呈无序排列,纤维膜覆盖,缺损区为纤维瘢痕组织。③兔骨缺损区放射学检查:于10周X射线拍片见骨缺损区无骨性连接,断端硬化,髓腔封闭,两侧断端有少量骨痂形成,骨痂形成不规则。结论:实验所建立的动物模型,具有骨不连的病理改变,符合骨不连的要求,反映了骨不连的一般特征,可以认为是一种可靠而实用的实验性骨不连动物模型。
BACKGROUND：There is lack of an objective and standardized animal model for assessing the therapeutic effect of physical and medication treatment on bone defect, the effectiveness of operation, as well as the role of bone substitute in the repairing of bone defects. DESIGN ：Verified study on the experimental model of bone nonunion in rabbits SETTING ： Department of Orthopaedics in Shenzhen people＇s Hospital MATERIALS ：Twenty common grade pure New Zealand rabbits of either gender were selected with body mass of （2.5±0.5）kg,aged 6 to 8 months. METHODS：This experiment was carried out at the experimental animal center of Shenzhen people＇s Hospital between May and August 1999. 1.5 cm bone segment （including periosteum）was cut off in the middle of foreann radius in 20 common grade pure NewZealand rabbits,the broken ends were covered with bone wax, 10 weeks later, the bone nonunion status was assessed by macropathological observation, pathohistological and X-ray examination. MAIN OUTCOME MEASURES：Observations on rahbit forearm radius defects by macropathological observation, pathobistological and X-ray examination. RESULTS ：Twenty rabbits（40 side radius）were enrolled in this study and all data were statistically analyzed. ①Macropathnlogical observation ：10 weeks later, bone defect region was found filled with fibrous cicatricial tissue without osseous connection ,bone wax was not absorbed, capitulum was ossified with medullary cavity blocked,a small amount of callus formed at both broken ends of fractural bone,length of bone defect ranged from 0.8 to 1.2 cm.②Pathohistological examination：broken medullary cavity was found blocked under optical microscope,chondrocyte and osteocyte could be observed arranging disorderly and covered with fibrous membrane,defect region was predominated by fibrous cicatricial tissue. ③X-ray examination：no osseous connection could be detected at defect region at week 10,broken end was ossified and medullary cavity was blocked,there was small amount of
出处
《中国临床康复》
CSCD
北大核心
2005年第30期214-215,共2页
Chinese Journal of Clinical Rehabilitation
