摘要
用ELISA方法测定95例SARS患者急性期、恢复期血清细胞因子水平,用流式细胞仪检测该组患者急性期、恢复期淋巴细胞亚群,并分析细胞因子水平和淋巴细胞亚群变化的关系。结果发现,IL-10和TGF-β在观察期(急性期和恢复期)持续升高,急性期CD4+和CD8+T细胞显著减少,恢复期患者外周血CD8+记忆T细胞减少36.78%。IL-10和TGF-β升高与淋巴细胞变化具有统计学相关。结果提示,SARS病毒感染抑制宿主的细胞免疫功能,引起急性期CD4+和CD8+T细胞显著降低和恢复期的免疫记忆细胞减少。而IL-10和TGF-β过表达可能在SARS免疫病理中起重要作用。
Serum levels of Th1/Th2 cytokines were assayed by ELISA and phenotypes of peripheral lymphocytes were analyzed by flow cytometry from 95 SARS-infected patients. The results showed that IL-10 and TGF-βwere continuously upregulated during the entirety of SARS development. CD4^ ＋ and CD8^＋ T lymphocytes decreased significantly in acute phase and CD3^＋ CD8^＋ CD45RO^＋ T lymphocytes were decreased by 36.78% in the convalescent patients. These indicated that SARS virus inhibited cellular immunity, especially CD8^＋ memory T lymphocytes over time. Prolonged overproduction of IL-10 and TGF-β may play an important role in the disease.
出处
《病毒学报》
CAS
CSCD
北大核心
2005年第5期358-363,共6页
Chinese Journal of Virology
基金
973计划SARS专项(2003CB514107)
教育部防治非典型科技攻关项目(教技司【2003】64号)
广东省防治非典型科技攻关项目(粤科社字【2003】263号)
广州市防治非典型科技攻关项目(2003Z3-E0451)
关键词
SARS
细胞免疫
激素
细胞因子
severe acute respiratory syndrome（SARS）
cellular immunity
corticosteroid
cytokine
