摘要
目的建立中药舒风口服液中5-O-甲基维斯阿米醇苷的含量测定方法。方法采用HPLC法,色谱柱:All-tima C18柱(150 mm×4.6 mm,5μm);流动相:甲醇-水(41∶59);检测波长:293 nm;流速:1 mL/min;柱温:40℃;保留时间:约13 min。结果5-O-甲基维斯阿米醇苷在0.12￣0.84μg之间呈良好的线形关系(r=0.99998);平均回收率为99.8%,RSD=2.05%(n=5)。结论该方法简便,快速,准确,专属性强,可作为舒风口服液的检验方法。
Objective To establish a method for the determination of 5-O-methylvisamminol in Shufeng oral liquid. Methods The sample was extracted with methyl and HPLC method was used. The chromatographic conditions were as follows：Alltima-C18 column（150 mm ×4.6 mnl,5 μm）with a mobile phase of methanol-water（41：59）, the detection wavelength at 293 nm and the flow rate being 1.0mL·min^-1. Results A linearity was obtained from 0. 12 μg to 0. 84μg of 5-O-Methyvisammioside in Shufeng oral liquid with a good eorrelation（r = O. 99998, n = 7）. The average recovery was 99.8 % and RSD = 2.05 % （n = 5 ）. Conclusion This method for determination of 5 - O - Methyvisammioside in Shufeng oral liquid is simple,sensitive, specific and accurate.
出处
《中药新药与临床药理》
CAS
CSCD
2005年第5期366-368,共3页
Traditional Chinese Drug Research and Clinical Pharmacology
