摘要
目的研究低剂量氯化汞在短时间内染毒致肾脏vero细胞DNA损伤的作用。方法常规培养vero细胞,通过MTT法制定出氯化汞染毒剂量,与氯化汞同时加入不同浓度的硫酸锌、亚硒酸钠以及硫酸锌+亚硒酸钠溶液,共同培养2 h后采用单细胞凝胶电泳法检测细胞的彗星尾长和彗星率。结果硫酸锌和亚硒酸钠,以及两者合用都具有拮抗氯化汞致vero细胞DNA损伤的作用,两者最佳组合是1.5 mg/L硫酸锌+1.0μmol/L亚硒酸钠。结论低剂量氯化汞短期内染毒可致肾脏vero细胞DNA损伤,硫酸锌和亚硒酸钠在体外可以有效拮抗该作用,两者共同作用效果更明显。这对进一步探索氯化汞肾脏毒性机制和防护具有一定的意义。
Objective To study the effect of zinc sulfate and sodium selenite which were used solely and together to contradict the DNA amage caused by mercury chloride in vero cells. Methods Vero cells were cultured with mercury chloride, at the same time zinc sulfate, sodium selenite and zinc sulfate together with sodium selenite were added respectively. The tail length and the rate of the comet cells were measured and calculated. Results zinc sulfate, sodium selenite and zinc sulfate together with sodium selenite had the effects of antagonism to DNA amage caused by mercury chloride in vero cells. The most suitable combination of the two substances should be intermediale dosage of zinc sulfate （1.5 mg/L） with intermediale dosage of sodium selenite （1.0 μmol/L）. Conclusion Mercury chloride have DNA amage in vero cells exposed to low dose of mercury chloride in short and sodium selenite which are used solely and together can reduce the DNA mage of in vero cells. term, zinc sulfate mercury chloride
出处
《苏州大学学报（医学版）》
CAS
北大核心
2005年第4期555-558,共4页
Suzhou University Journal of Medical Science
关键词
氯化汞
亚硒酸钠
硫酸锌
单细胞凝胶电泳试验
DNA损伤
mercury chloride
sodium selenite
zinc sulfate
single cell gel electrophoresis（SCGE）
DNA amage
