摘要
为研究重组β琼脂糖酶(AgaA7)基因的表达产物及其生物活性,根据GeneBank中β琼脂糖酶(AgaA7)基因的编码序列合成此基因后,将其克隆到表达载体PQE30中,测序结果证明合成基因序列正确,未改变读码框.然后将此表达载体转入E.coliM15中进行诱导表达.纯化后的表达产物经SDS-PAGE检测其相对分子质量并鉴定其生物活性.结果表明:其相对分子质量约为5.0×104,与预期相符,具有生物活性.
In order to study the exprssion product of recombinant β-agarase （AgaA7） gene and its bioactivities, the full β-agarase（AgaA7） sequence is synthesized according to the GeneBank. Then it is inserted into expression vector PQE30. Its sequence is verified and the reading frame is proved to be correct. The recombinant plasmid is transformed into E. coli M15. The molecular mass of the purified expression product is examined by SDS-PAGE and its bioactivities are proved.
出处
《北京师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2005年第4期415-419,共5页
Journal of Beijing Normal University(Natural Science)
