摘要
目的:探讨乳癌组织中凋亡调控因子Caspase-9的表达及意义。方法:选取存档蜡块中的乳腺浸润性导管癌38例(其中伴有腋窝淋巴结转移者30例)、导管内癌8例及乳腺纤维腺瘤10例,进行Caspase-9免疫组织化学和原位杂交检测。结果:Caspase-9 mRNA及其蛋白在乳癌中呈现高表达。Caspase-9蛋白和mRNA在乳腺浸润性导管癌、乳腺导管内癌和乳腺纤维腺瘤组间表达差异有统计学意义,乳腺浸润性导管癌Caspase-9蛋白和mRNA表达显著高于乳腺纤维腺瘤。乳腺浸润性导管癌中Caspase-9蛋白表达和mRNA阳性率在淋巴结转移组与无淋巴结转移组的差异无统计学意义。结论:Caspase-9异常表达与乳癌的发生密切相关,可以作为乳癌预后判断的指标之一。
Aim： To study the expression of apoptotic effector Caspase-9 in the breast cancer tissue. Methods： Thirty-eight cases of invasive ductal breast cancer, 8 cases of intraductal cancer and 10 cases of breast fibroadenoma were included in this study. Immunohistochemical ABC and in situ hybridization staining methods were used to examine the expression of Caspase-9 protein and mRNA in all tissue. Results： There were significant difference in the positive rates of Caspase-9 protein and mRNA among the invasive breast cancer, intraductal cancer and breast fibroadenoma, and the positive rates of Caspse-9 protein and mRNA in the invasive breast cancer were significantly higher than those in breast fibroadenoma. The positive rates of Caspase-9 protein and mRNA had no significant differences in cases with lymph node metastasis and those without in invasive breast cancer. Conclusion： It is suggested that the higher expression of Caspase-9 is closely correlated with the carcinogenesis of breast cancer and can be used as a diagnostic marker.
出处
《郑州大学学报（医学版）》
CAS
北大核心
2005年第5期810-812,共3页
Journal of Zhengzhou University(Medical Sciences)
基金
河南省科技攻关基金资助项目0324410036
