摘要
目的:观察慢性轻度不可预见的刺激对大鼠血清皮质酮和行为学的影响及睡眠剥夺的调剂作用。方法:实验于2003-10/11在汕头大学医学院完成。选择32只SD大鼠,随机分为4组,即正常对照组,抑郁模型组,睡眠剥夺组和大平台对照组,每组8只。抑郁模型组,睡眠剥夺组和大平台对照组均采用慢性轻度不可预见性的应激抑郁模型。睡眠剥夺组在抑郁模型的基础上,采用小平台水环境法进行72h的睡眠剥夺。大平台对照组中平台的直径大于睡眠剥夺组,为18cm,余与异相睡眠剥夺组的环境相同。以旷场实验研究睡眠剥夺对大鼠行为学的影响;采用酶联免疫吸附试验检测大鼠血清皮质酮的含量。结果:纳入动物32只,进入结果分析32只,无脱失值。①各组大鼠旷场行为得分:抑郁模型组,睡眠剥夺组和大平台对照组造模后的总活动量比造模前降低(17.25±4.03,99.25±5.98;12.13±3.01,90.00±6.04;15.50±3.27,96.25±7.06);睡眠剥夺72h后,睡眠剥夺组和大平台对照组的总活动量比造模后升高(71.00±5.84,12.13±3.01;53.50±9.42,15.50±3.27)。②慢性轻度不可预见刺激使大鼠血清皮质酮提高,睡眠剥夺使抑郁模型大鼠的血清皮质酮下降,正常对照组为(37.62±6.63)μg/L,抑郁模型组高于正常对照组犤(91.60±17.63)μg/L犦,睡眠剥夺组和大平台对照组低于正常对照组犤(3.43±0.90),(3.23±0.68),(37.62±6.63)μg/L,P<0.01犦,且睡眠剥夺组和大平台对照组均低于抑郁模型组(P<0.01)。结论:慢性轻度不可预见刺激可使大鼠血清皮质酮提高,即下丘脑-垂体-肾上腺轴功能亢进,并出现抑郁样行为;睡眠剥夺使抑郁模型大鼠的血清皮质酮下降,即抑制大鼠下丘脑-垂体-肾上腺轴的兴奋性,改善抑郁模型大鼠的抑郁表现。
AIM： To observe the chronic mild unpredictable stress on serum corticosteron and praxiology as well as the regulation role of sleep deprivation METHODS： The experiment was conducted in Medical College of Shantou University from October to November 2003. Totally 32 SpragueDawley rats were randomly divided into four groups： normal control group, depression model group, sleep deprivation group and tank control group with 8 rats in each group . Chronic mild unpredictable stress depression models were used in the depression model group, sleep deprivation group and tank control group. Based on the depression model, the rats in the sleep deprivation group were deprived their sleep for 72 hours on plowerpot. Diameter of plowerpot in the tank control group was 18 cm. Other environment was the same as that in the sleep deprivation group. An open field test was repeated three times to evaluate the depressive-behavior during the experiment. Concentration of serum corticosterone was measured by enzyme-linked immunosorbant assay （ELISA）. RESULTS： All the 32 rats involved in this experiment entered result analysis with no loss. ① The results of open field tests were as followings： the scores of depression model, sleep deprivation and tank control rats were reduced after model establishing （17.25±4.03,99.25±5.98;12.13±3.01,90.00±6.04; 15.50±3.27,96.25±7.06）;72 hours after sleep deprivation, the scores of sleep deprivation and tank control rats was increased than that after model cstablishing （71.00±5.84,12.13±3.01; 53.50±9.42,15.50±3.27）.②The unpredictable mild stressors enhanced serum corticosterone in rats, while sleep deprivation reduced serum corticosterone in depression model rats. The concentration of serum eorticosterone of normal control rats was （37.62±6.63）μg/L, the concentrations of serum corticosterone in the depression model group [（91.60±17.63）μg/L] was higher than that in the normal control group, the concentrations of serum corticosterone in the sleep deprivation gr
出处
《中国临床康复》
CAS
CSCD
北大核心
2005年第32期156-157,共2页
Chinese Journal of Clinical Rehabilitation
基金
广东省自然科学基金资助项目(04020238)~~
