摘要
目的:探讨大鼠胰腺胚胎发育后期胰岛形成及β细胞功能完善相关的基因表达调控.方法:采用高密度寡核苷酸芯片(A ffym etrix芯片)对孕15.5(E15.5)和E18.5胰腺进行基因转录水平分析并用RT-PCR验证基因在大鼠胰腺E12.5,E15.5,E18.5,初生和成年这五个不同发育时期的表达情况.结果:在差异或特异表达的基因中与胰腺细胞分化相关的转录因子和信号分子表达均下调,而与胰岛结构形成及β细胞代谢功能完善相关的基因表达上调,其中细胞黏附分子Mesothelin是在E18.5特异表达的基因,RT-PCR亦显示Mesothelin在大鼠胰腺胚胎发育后期特异性高表达.结论:Mesothelin可能对胰岛结构的形成和功能的完善及维持有重要作用.
AIM： To investigate the molecular mechanism involved in the islet formation and maturation of metabolic regulation by β cells. METHODS： The expression patterns of pancreas at embryonic day 1.5.5 （E15.5） and E18.5 were compared using the GeneChip Rat Expression Set 230 A. Based on the expression profiles of genes, the expression of mesothelin at different stages of rat pancreatic development was detected by RT-PCR. RESULTS. Out of 1319 genes of different expressions, the transcription factors and signal components related to cell differentiation were downregulated at the mRNA level while the genes related to islet formation and the function of metabolic regulation were upregulated. Mesothelin was specifically expressed at E18.5 pancreas, which was confirmed by RT-PCR. CONCLUSION： It is from El5.5 to El8.5 that the islet is gradually formed and undergoes further remodeling and maturation. Mesothelin may be a novel regulator of the islet formation and maturation.
出处
《第四军医大学学报》
北大核心
2005年第17期1570-1572,共3页
Journal of the Fourth Military Medical University
基金
江苏省科委应用基础项目(BK2001119)
