摘要
目的:观察凋亡相关基因Survivin和Caspase-9在胃癌组织中的表达以及它们与胃癌发生的关系。方法:采用RT-PCR方法检测Survivin和Caspase-9在30例胃癌组织、10例正常胃组织标本中的表达。结果:30例胃癌组织中20例SurvivinmRNA表达阳性,10例正常胃组织无SurvivinmRNA表达;30例胃癌组织和10例正常胃组织均有Caspase-9mRNA表达,20例SurvivinmRNA表达阳性的胃癌组织Caspase-9mRNA表达水平与10例SurvivinmRNA表达阴性的胃癌组织Caspase-9mRNA表达水平无明显差别,30例胃癌组织Caspase-9mRNA表达水平明显低于10例正常胃组织。结论:Survivin在胃癌组织中的表达明显升高,Caspase-9表达明显降低。Survivin表达升高及Caspase-9表达降低是胃癌发生的重要因素之一。
Objective： To investigate expression of apoptosis-associated genes survivin and Caspase-9 in gastric cncer tissues and its＇ relationship with tumorigenesis of gastric cancer. Methods： RT-PCR for survivin and Caspase-9 expression was conducted on 30 gastric cancer tissues and 10 gastric normal tissues. Results： Survivin was expressed in 20 of 30 （66.7%） gastric cancer tissues. In contract, 10 gastric normal tissues did not express survivin. Although all of 30 gastric cancer tissues and l0 gastric normal tissues express Caspase-9, expression value of Caspase-9 in 30 gastric cancer tissues was strongly decreased than that in l0 normal gastric tissues （P〈0.01）, and there wasn＇t signifi- cant difference in expression value of Caspase-9 between 20 survinvin-positive and 10 survinvin-negative gastric cancer tissues. Conclusions： The expression of survivin in gastric cancer tissues is significantly increased and Caspase-9 is significantly decressed, overexpress of survivin and low express of Caspase-9 are both one of the important factors that cause tumorigenesis of gastric cancer.
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
2005年第17期969-972,共4页
Chinese Journal of Clinical Oncology
基金
重庆市自然科学基金资助(编号:6832)
关键词
胃癌
基因
凋亡
Gastric cancer Genes Apoptosis
