摘要
参考GenBank中伪狂犬病病毒(PRV)gE基因的序列设计了1对引物,对PRV粤A株gE基因进行了PCR扩增,PCR产物克隆到PMD18_T载体。对重组质粒进行限制性内切酶分析和基因测序,证实了克隆片段的可靠性,并与2株不同来源的毒株进行了同源性分析和比较。
A pair of primers were designed according to the sequences published in the GenBank in order to amplify the gE gene of the pseudorabies virus YA strain. The gE gene was isolated by polymerase chain reaction, and subsequencely cloned into the PDM18-T vector. The recombinant plasmid was identified by restriction enzyme analysis and nucleotide sequencing, which completely proved its validity. The homology of the gE gene of PRV YA strain with two different strains were studied.
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2005年第5期352-355,共4页
Chinese Journal of Preventive Veterinary Medicine
基金
广东省科技厅重大攻关项目(2003A20403)资助
关键词
伪狂犬病病毒
GE基因
克隆
序列分析
pseudorabies virus
gE gene
cloning
nucleotide sequencing
