摘要
为探讨5-羟色胺和神经肽Y在锯缘青蟹脑内的存在与否和分布状况,应用免疫细胞化学技术,在光学显微镜下观察5-羟色胺和神经肽Y阳性细胞和神经髓质的形态和分布。结果表明:在锯缘青蟹脑中共12个胞体群和11个神经髓质中,前脑和中脑中有4个细胞群和6个神经髓质检出5-HT免疫阳性反应;中脑和后脑中有7个细胞群和3个神经髓质具有NPY免疫阳性反应。5-羟色胺和神经肽Y在锯缘青蟹脑内的特异性分布,为其参与神经生理活动提供了形态学证据。
To explore the locations of serotonin （5-HT） and neuropeptide Y （NPY） in the brain of Scylla serrata, immunocytochemical method of Strept Avidin-Biotin-Complex was applied to observe the immunoreaction to two antibodies. Among all the 12 somal clusters and 11 neuropils of the brain in S. serrata, 5-HT-like immunoreactivity occurs in 4 somal clusters and 6 neuropils in the protocerebrum and deutocerebrum; while NPY-like immunoreactivity is present in 7 somal dusters and 3 neuropils in the deutocerebrum and tritocerebrum. The specific distribution patterns of these two immunoreactive substances may thus provide morphological proofs for their different neurophysiological functions.
出处
《水产学报》
CAS
CSCD
北大核心
2005年第4期441-446,共6页
Journal of Fisheries of China
基金
FundedbytheNationalNaturalScienceFoundationofChina(No.30300269)
