摘要
ＭＮＮＧ所致哺乳类细胞非定标性突变的时相分析１孙雪敏１余应年张小山陈星若（浙江医科大学病理生理学教研室及医学分子生物学实验室，杭州３１００３１；１杭州医学高等专科学校，杭州３１００１２）非定标性突变（ｎｏｎｔａｒｇｅｔｅｄｍｕｔａｔｉｏｎ）为原核细胞...
On the basis of the evidence for nontargeted mutagenesis in mammalian cells acquired in our laboratory, the mechanism of mutation was studied. lntact shuttle vector pZ189 DNA was allowed to replicate in monkey kidney vero cells at various times, i.e. 3, 6, 12, 24, 36 and 48 h after the cells wertreated with 0 2 μmol·L -1 N methyl N′ nitro N nitrosoguanidine (MNNG). The E. coli MBM 7070 was transformed with replicated plasmid, and those with mutations in the supF tRNA gene were identified. DMSO, the solvent of MNNG was served as control simultaneously at each time group. Compared with the control, the increasing mutation frequency was found at 6 h and 12 h after MNNG exposure, with the relative ratio of MNNG induced and spontaneous mutation rate was 4 42 (P<0 01) and 5 44 (P<0 01) respectively. The dynamic behavior of mutation frequency accorded with the metabolic characteristic of proteins and suggested that newly synthetic proteins would be required in the process of nontargeted mutation formation in mammalian cells.
出处
《中国药理学与毒理学杂志》
CAS
CSCD
北大核心
1996年第1期77-78,共2页
Chinese Journal of Pharmacology and Toxicology
基金
国家和浙江省自然科学基金
关键词
癌
细胞
非定标性突变
甲基硝基亚硝胍
时相分析
mutation, nontargeted
N methyl N′ nitrosoguanidine
shuttle vector
phasic analysis.
