摘要
目的研究乐果对小鼠骨骼肌的细胞毒性和凋亡的诱导效应,探讨急性有机磷农药中毒中间型综合征(IMS)肌无力发生的细胞学基础。方法以35,350,3500μmol/L乐果浓度接触3h及350μmol/L乐果浓度接触3,9,24h,观察乐果对小鼠骨骼肌细胞形态、细胞活力以及对细胞凋亡率的影响。结果乐果具有明显的细胞毒性,使乳酸脱氢酶(LDH)漏出增加和细胞存活率明显下降,并具有明显的剂量依赖关系(r=0.684,P<0.01)和时间依赖关系(r=0.955,P<0.01);乐果处理能诱导明显的骨路肌细胞凋亡形态学变化及凋亡率增加。结论乐果对离体骨骼肌细胞具有明显细胞毒性,并诱导骨骼肌细胞的凋亡,这可能在IMS的发病过程中发生作用。
Objective To investigate the cellular mechanism of muscular weakness in the Intermediate Myasthenia Syndrome（ IMS）following acute organophosphate poisoning. Cytotoxicity of dimethoate and its effects on the apoptosis were studied in mouse skeletal muscle cells. Methods Skeletal muscle ceils were incubated with 35350 and 3 500μmol/L concentrations of dimethoate for 3 h or incubated for 3, 9, 24 h with 350μmol/L concentrations. The morphological changes of cultured cells were observed; cell viability were assessed with typan blue dye and apoptotic cells were detected by flow cytometer （FCM）;the activity of lactic dehydrogenase（LDH） was measured by colorimetry method. Results There were significantly increased in a dose-dependent and time-dependent manner（r=0.821, r =0.955, P〈0.01）, and the cell viability also decreased, and apoptotic rate were increased after exposure of dimethoate. Conclusion Dimethoate has significantly cytotoxicity and may induce apoptosis on skeletal muscle cell in vitro. These effects may play an important role in the etiology of IMS.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2005年第9期1105-1106,共2页
Chinese Journal of Public Health
