摘要
背景:光学记录技术是以电压敏感染料为介质,以硅光电二极管转换技术为特点的新型电生理检测方法,有助于分析复杂的神经结构中膜电位变化的时间-空间分布。目的:使用光学记录的方法观察小鼠脑干听神经电刺激诱发冲动的时间-空间分布,并分析抑制性神经递质γ-氨基丁酸和γ-氨基丁酸A受体拮抗剂荷包牡丹碱对听觉诱发冲动的影响。设计:随机对照实验。单位:解放军总医院老年医学研究所,日本关西医科大学耳鼻喉科。材料:实验于2002-05/11在日本关西医科大学耳鼻喉科实验室完成。选取出生后0～5d的ddy/ddy小鼠100只,清洁级,雌雄不拘。方法:将全部动物麻醉后断头处死,在冷冻条件下制作有活性的脑干切片。脑片一侧与无损伤的听神经根残端连接。将脑片置于硅胶铺底的有机玻璃平皿中,用直径30μm钨丝固定。用钨丝电极刺激脑片一侧的听神经根残端,在耳蜗核、前庭核记录诱发反应。对照情况下使用正常的人工脑脊液作为灌流液;灌流液中加入50μmol/Lγ-氨基丁酸的人工脑脊液代替正常人工脑脊液孵育脑片20min后,记录γ-氨基丁酸对脑干听觉通路诱发信号的影响;灌流液中加入50μmol/Lγ-氨基丁酸和200μmol/L荷包牡丹碱的人工脑脊液代替含50μmol/Lγ-氨基丁酸的人工脑脊液孵育脑片20min后,记录荷包牡丹碱对脑干听觉通路诱发信号的影响。刺激条件为正相矩形波脉冲,电流强度5mA,持续时间5ms,频率0.1Hz,电刺激起始时间被设置为89.9ms。脑片染色采用电压敏感染料NK3041,使用16×16像素的硅光电二极管阵列设备记录刺激听神经所诱发的光学信号。主要观察指标:①主要结局;电刺激听神经诱发的脑干光学信号及其特点。②次要结局:γ-氨基丁酸和荷包牡丹碱光学反应记录结果。结果:实验纳入ddy/ddy小鼠100只,中途死亡56只,共44只进入结果分析。①电刺激听神经诱发�
BACKGROUND： Optical mapping technique is a novel electrophysiological detection method in which voltage-sensitivity dye is medium and silicon photoelectrical diode transforming technology is characteristic, used for analyzing the spatial-temporal distribution of membrane potential in complex neural system. OBJECTIVE： To observe the spatial-temporal changes of brainstem auditory electro-stimulation evoked potential by using optical mapping technol- ogy, so as to probe into the influence of γ--Aminobutyric acid （GABA） and γ-GABA receptor antagonist-bicuculline （BM1） on auditory evoked potential. DESIGN： Randomized controlled study. SETTING： Aging Medicine Research Institute of Military General Hospital and E.T.N Department of Japanese Kansai Medical University. MATERIALS＇This study was conducted at E.T.N Department of Japanese Kansai Medical University from May to November 2002. Totally 100 ddy/ ddy rats, with age of 0-5 days, clear grade, either gender were selected. METHODS： All rats were put to death after cryo-anaesthetized, andbrainstem was cut into slices under frozen state so as to remain activity. One side of brainstem slice was connected with the residual end of untraumatic auditory nerve, and slices were put on organic glass plate with the bottom covered with siliac gel and fixed by tungsten filament of 30 μm wide. The residual end of untraumatic auditory nerve was stimulated by tungsten electrode, meanwhile the evoke potentials were recorded at cochlea nuclei and vestibule nuclei. In control group slices were incubated in artificial CSF for 20 minutes, which added with 50 μmol/L γ-GABA in experimental group for observing the influence of γ-GABA on brainstem auditory evoked signals; or alternatively incubated with 50 μmol/L g-GA- BA and 200 μmol/L for 20 minutes for observing the influence of BMI on brainstem auditory evoked signals. Stimulation was positive rectangle-shape impulse with electric current of 5 mA and frequency of 0.1 Hz, lasting period of 5 ms, the onset time of
出处
《中国临床康复》
CSCD
北大核心
2005年第29期219-221,i0003,共4页
Chinese Journal of Clinical Rehabilitation
