摘要
目的探讨葡聚糖氧化铁磁性纳米颗粒(DMN)的制备对其有效粒径和比饱和磁化强度的影响。方法采用化学共沉淀法制作DMN,通过丙烯葡聚糖凝胶S300HR色谱和离心法分离DMN,用透射电镜、粒度分析仪和磁化强度分析仪检测DMN,研究制备过程中葡聚糖用量、铁盐含量、氨水浓度、搅拌速度、离心速度、Fe3+/Fe2+摩尔比、反应温度等条件对DMN的有效粒径、比饱和磁化强度的影响。结果不同制备条件影响着DMN有效粒径和比饱和磁化强度的大小。DMN直径最小为3nm,有效粒径介于42.7～189.1nm,比饱和磁化强度介于0.16～0.38emu/g。结论制备反应条件对DMN有效粒径和比饱和磁化强度的影响符合化学共沉淀反应的一般规律,可以用沉淀反应的理论指导DMN的制备,为DMN作为基因和药物纳米载体的研究提供了实验数据。
Objective To investigate the effect of preparation conditions on the effective diameter and saturation magnetization （Ms） of dextran magnetic iron oxide nanoparticles （DMN）. Methods DMN were prepared by chemical co-precipitation method. DMN were separated by gel filtration chromatography on Sephacryl-300HR and centrifugation, characterized by TEM, laser scattering system and Vibrating Sample Magnetometer Signal Processor. During the course of preparation of DMN, the effects of preparation conditions, such as dextran concentration, amount of ironic salts, concentration of ammonium hydroxide, stirring, velocity, cent rifugation velocity, Fe^3＋/Fe^2＋ molar ratio, reaction temperature, etc. on the effective diameters and Ms of DMN were studied. Results Different preparation conditions could influence the effective diameter and Ms of DMN. The minimum diameter of DMN was 3nm. The effective diameter was between 42.7-189.1nm. Ms was between 0.16-0.38 emu/g. Conclusion The effect of preparation conditions on the effective diameters and Ms of DMN accorded with general regularity of the method of chemical co-precipitation. The preparation of DMN was instructed by the theory of chemical co-precipitation, which provided the experimental data for the feasibility of DMN as gene and drug nanometer carrier.
出处
《华中科技大学学报（医学版）》
CAS
CSCD
北大核心
2005年第4期482-485,共4页
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
基金
国家自然科学基金资助项目(No.30271300)
关键词
葡聚糖
氧化铁磁性纳米颗粒
有效粒径
比饱和磁化强度
dextran
magnetic iron oxide nanoparticles
effective diameter
saturation magnetization
