摘要
目的了解在KG1诱导来源的树突样细胞(Dendritic-like cells, DLCs)中DC特异性C型凝集样受体DC-SIGN的表达.方法采用佛波酯(PMA)和离子霉素(Ionomycin)诱导KG1细胞为DLCs,采用Western免疫印迹、RT-PCR和流式细胞仪检测KG1和KG1来源的DLCs不同诱导时期DC-SIGN的表达.结果形态学方法确定成功诱导了KG1来源的DLCs,KG1细胞和其来源的DLCs均有DC-SIGN的表达,且当KG1诱导为DLCs细胞后DC-SIGN表达增强,尤其在DLCs的早期成熟阶段(诱导12～24 h).结论 KG1细胞和其来源的DLCs上均有DC-SIGN的表达,且在诱导为DLCs后DC-SIGN的表达增强,为进一步研究DC-SIGN介导的抗原呈递途径奠定了基础.
Objective To investigate the expression of DC-specific C type-like cells recoptor DC-SIGN in KG1 cells and KG1 derived dendritic-likecells （DLCs）. Methods KG1 was stimulated by phorbol ester（PMA） in combination with ionomycin. Expression of DC-SIGN was detected by western blotting, RT-PCR, and flow cytometry in KG1 and KG1 derived DLCs of different stimulation periods. Results KG1 cells were derived to DLCs successfully according to morphology of cells. DC-SIGN was expressed in KG1, and the expression was enhanced in KG1 derived DLCs. DC-SIGN expression in KG1 derived DLCs increased rapidly at early maturation stage （treatment by PMA and ionomycin in 12-24h）. Conclusion DC-SIGN is expressed in KG1 and KG1 derived DLCs, and the expression will increase after treatment by PMA and ionomycin, which provides an cell model for further study of antigen presentation mediated by DC-SIGN.
出处
《免疫学杂志》
CAS
CSCD
北大核心
2005年第5期397-399,共3页
Immunological Journal
