摘要
目的对尖锐湿疣中人乳头瘤病毒(HPV)cp6108进行序列研究。方法应用TA克隆后测序和PCR产物直接测序进行HPV cp6108的基因序列测定。结果 5例HPV cp6108阳性的尖锐湿疣的模板DNA经TA克隆后测序和PCR产物直接测序结果显示,两种测序结果一致,BLAST比对结果显示,与基因库中HPV cp6108提交序列有99％的一致性,排在比对结果第1位,所有标本的cp6108的序列与网上提交序列对比后发现均有3处相同的密码子发生改变(以起始密码子GCA之G为第1位)：第210位核苷酸G(鸟嘌呤,)突变为A(腺嘌呤),导致第70位密码子GAG→GAA,发生同义突变；第229位核苷酸G(鸟嘌呤)突变为A(腺嘌呤),导致第77位密码子GAT→AAT,编码的氨基酸由天冬氨酸(Asp,D)变为天冬酰氨(Asn,N),即发生D77N错义突变；第346位核苷酸A(腺嘌呤)突变为C(胞嘧啶),导致第116位ACT→CCT,编码的氨基酸由苏氨酸(Thr,T)变为脯氨酸(Pro,P),即发生T116P错义突变。结论与基因库提交序列相比,本研究检测到的HPV cp6108序列中有3处突变,其中G70A为同义突变,D77N和T116P为错义突变。
Objective To study L1 gene sequence ot HPV cp6108 from 5 cases of conoyloma acuminata. Methods T-A cloning and direct sequencing of PCR product were used. Results The L1 gene sequences of HPV cp6108 from 5 specimens were presented with the homology of 99% to reference sequence in GenBank. A total of 3 gene mutations were found. including a nonsense mutation of GTOA. a missense mutation of D77N, and a missense mutation of T116P. Conclusions In comparison with the sequence in GenBank, at least 3 gene mutations of HPV CP6108, i.e. one nonsense mutation of G70A and missense mutations of D77N and T116P, are found in the present study.
出处
《中华皮肤科杂志》
CAS
CSCD
北大核心
2005年第9期543-545,共3页
Chinese Journal of Dermatology
