摘要
目的建立检测8种常见皮肤分枝杆菌的PCR-RFLP方法。方法①用通用引物对分枝杆菌的热休克蛋白hsp65基因序列进行PCR扩增：②用限制性内切酶BstEⅡ和HaeⅢ对扩增产物进行消化,根据所得片段确定各分枝杆菌特征性的酶切图谱,从而对分枝杆菌进行鉴别。结果 8种常见皮肤分枝杆菌感染标准株(鸟、胞内、堪萨斯、结核、瘰疬、海、偶发、龟分枝杆菌)的酶切图谱与文献报道一致。结论 PCR-RFLP方法可用以检测鉴定皮肤分枝杆菌。
Objective To develop a PCR-RFLP method for the identification of eight mycobacterial species. Methods PCR was performed targeting the gene encoding 65-kDa heat shock protein which was common to all myeobaeteria. Two restriction enzymes, BstE Ⅱ and Hae Ⅲ, were used to digest the PCR produets, and specific restriction patterns of different mycobacteria were obtained. Results The specific restriction patterns of different mycobaeteria were identical to the data previously reported. Conclusion We could differentiate M. avium, M. intracellulare, M. kansasii, M. tuberculosis, M. scrofulaceum, M. marinum, M. fortuitum and M. chelonae in one experiment by PCR-RFLP.
出处
《中华皮肤科杂志》
CAS
CSCD
北大核心
2005年第9期533-535,共3页
Chinese Journal of Dermatology
