摘要
以马立克氏病病毒超强毒(RB1B株)UL49为靶基因,利用计算机辅助设计UL49基因特异的siRNA,再定向克隆至pSilencerTM2.1U6neovector载体中构建siRNA表达重组体,转化DH5α菌株,提取质粒酶切鉴定后,进行测序分析,证实为所需序列。以上结果表明,针对马立克氏病超强毒UL49基因的siRNA表达质粒已构建成功。
To seek new gene therapy method of Marek＇s disease, siRNAs aimed to UL49 gene of Marek＇s disease virus RB1B strain were designed and directionally cloned into pSilencer6TM 2. 1-U6 neo vector, and transformed into E. coli DH5α strains. The combinant plasmids were identified by restriction enzyme and sequence analysis. Finally, the plasimds contained siRNA expressing cassette were obtained successfully and made it possible to search gene therapy method of the Marek＇s disease.
出处
《南京农业大学学报》
CAS
CSCD
北大核心
2005年第3期137-139,共3页
Journal of Nanjing Agricultural University
基金
上海市启明星计划项目(03QC14041)
