摘要
目的通过系统测定大鼠肝硬化发展过程中肝细胞凋亡的变化,与常规肝功能测定结果比较,了解上述指标与肝储备功能的关系。方法取Wistar大白鼠90只分为3组,分别为9周组、12周组、15周组,每组30只,采用复合因素(CCl4,乙醇)制备大鼠肝硬化模型;另取Wistar大白鼠30只随机分为3组,每组10只,作为与实验组相对应的对照组,代之等量生理盐水。分别于9周、12周、15周末处理9周组、12周组、15周组大鼠,同期处理相应对照组。血液标本作常规肝功检测;肝组织均作石蜡包埋,连续切片作HE染色、细胞凋亡检测。结果在大鼠肝硬化发展过程中HE染色显示9周组、12周组、15周组分别符合肝纤维化Ⅲ、Ⅳ期、肝硬化早期,肝细胞凋亡指数(AI)在9周组(7.38±1.71)、12周组(14.81±2.99)、15周组(26.55±7.94)时高于同期对照组(P<0.05)。结论①肝细胞凋亡指数随着肝硬化程度的加重而升高;②测定肝细胞凋亡指数对预测肝硬化患者的肝储备功能可能具有重要的意义。
Objective By studying the dynamic alterations of hepatocellular apoptosis and common liver function in rats with carbon tetrachloride-induced liver cirrhosis,to explore the relationship between this index and liver reserve function. Methods 90 male Wistar rats randomly divided into 3 groups,9 W,12 W,15 W were experimental groups;30 male Wistar rats randomly divided into 3 groups, were corresponding control groups,developing models of rat liver cirrhosis with complex factors （CCl4, alcohol）, and the control group with saline. The blcod and the liver tissue were taken for measures of the group 9 W, 12 W, 15 W after 9,12,15 weeks , and those of the corresponding control group were killed at the same time. The blood was taken for the determination of common hepatic function. The livers were taken for pathologic examination, in situ TUNEL.Results The expression of hepatocellular apoptosis in experimental groups was significantly higher than control group, 9 W（7.38 ± 1.71）, 12 W（14.81 ± 2.99） and 15w（26.55 ± 7.94） .Conclusion The rates of hepatocellular apoptosis may be has important meaning in predicting liver reserve function.
出处
《广西医学》
CAS
2005年第8期1131-1133,共3页
Guangxi Medical Journal
基金
广西壮族自治区自然科学基金资助项目(桂科自:0229028)
关键词
肝硬化
细胞凋亡
肝功能
Liver cirrhosis
Apoptosis
Liver function
