摘要
目的探讨尿胱蛋白酶抑制剂C(cystatinC,cysC)水平对窒息新生儿肾小管损伤的早期诊断价值。方法按Apgar评分标准将入选新生儿3天以内的尿标本分为正常对照、轻度窒息和重度窒息3组。尿cysC和尿视黄醇结合蛋白(RBP)用酶联免疫吸附法测定,Ucr用全自动散射率比浊法测定,尿IgG、Alb、α1-MG用放射免疫法测定。结果(1)轻度窒息组尿IgG和Alb值与对照组相比无统计学差异(P>0.05),而α1-MG、RBP和cysC非常显著高于对照组(P<0.01=;重度窒息组,尿IgG、Alb、α1-MG、RBP和cysC较对照组和轻度窒息组均有显著升高(P<0.01=。(2)轻度窒息组尿α1-MG异常率为54.55%显著高于IgG、Alb异常率(分别为27.27%和31.82%)(P<0.05=;尿cysC、RBP异常率分别为68.18%和72.73%非常显著高于尿IgG、Alb异常率(P<0.01=;尿cysC、RBP异常率显著高于尿α1-MG异常率(P<0.05=。重度窒息组尿,α1-MG、cysC、RBP的异常率分别达90%、100%和100%,显著高于尿IgG、Alb异常率(55%、65%)(P<0.05=;尿cysC、RBP异常率均显著高于尿α1-MG异常率(P<0.05=;尿cysC异常率与尿RBP异常率无显著性差异(P>0.05)。(3)尿cysC、RBP与α1-MG间有良好直线相关性(r分别为0.803和0.831,P<0.001=。结论尿cysC可作为反映窒息新生儿肾小管损伤的早期诊断指标,其敏感性优于尿α1-MG,与尿RBP相似。
Objective： To evaluate the diagnostic value of urinary cystatin C for the postasphyxial renal tubule injury in neonates. Methods： Based on the standard of calculating Apgar score, 61 urine samples which were collected within the first three days of life were divided into control group , mild asphyxia group and severe asphyxia group. Urinary cys C and RBP levels were measured by enzyme - linked immunoabsordent assay（ ELISA）. Ucr levels were measured by autovelocity scatter tubidimetry. Urinary IgG, Alb, α1 - MG levels were measured by radioimmunoassay （RIA）. Results： Results showed that： （1）Urinary α1- MG, RBP , cys C levels in mild asphyxia group were significantly higher than those in control group（ P 〈 0.01 ）, while urinary IgG, Alb, α1 - MG, RBP, cys C levels in severe asphyxia group were significantly higher than those in the two other groups（ P 〈 0.05）. （2） Both in mild asphyxia group and in severe asphyxia group, the abnormality rates of urinary α1- MG, RBP, cys C （54.55 % and 90% ,68%. 18% and 100%, 72.73% and 100% ） were significantly higher than those of urinary IgG and Alb （27.27% and 55% ,31.82% and 65%, P 〈 0.05）. Among the above, the abnormality rates of urinary cys C, RBP were significantly higher than those of urinary α1- MG, furthermore, there were an approximate abnormality rates between urinary eys C and RBP （P 〉 0.05）. （3）There was a significant correlation between urinary eys C, RBP and urinary α1 - MG（ r = 0.803 and 0.831, P 〈 0.00.1 ）. Conelusion ： Urinary eys C ean be eonsidered as a marker of renal tubule damage of asphyxial neonates, whose diagnostie value was better than that of urinary α1- MG and equal to that of RBP.
出处
《中国优生与遗传杂志》
2005年第8期33-35,共3页
Chinese Journal of Birth Health & Heredity
