摘要
目的探讨临产后母血、羊水中前列腺素E2(PGE2)浓度的变化与胎盘滋养层细胞bcl-2蛋白表达的关系。方法53例足月妊娠妇女分成两组,其中临产组28例,未临产组25例。用放射免疫法测定孕妇母血、羊水中PGE2的浓度,用免疫组化(IHC)法测定胎盘滋养层细胞bcl-2的蛋白表达。结果(1)临产组母血、羊水中PGE2水平均高于未临产组(P<0.05)。(2)临产组胎盘滋养层细胞bcl-2的蛋白表达明显低于未临产组(P<0.05)。(3)母血、羊水中PGE2的浓度变化与bcl-2的蛋白表达呈负相关。结论PGE2及bcl-2介导的胎盘滋养层细胞凋亡在启动分娩发动过程中具有重要作用;且PGE2可能影响bcl-2的表达。
Objective：To investigate the changes of the concentrations of ProstaglandinsE2 （PGE2） in maternal blood and amnlotic fluid on laber onset, and its influence to the expression of bcl - 2 proteins in placental trophoblastic cells . Methods：Fifty - three women on term pregnancy were divided into two groups. One group on the time of labor onset were twenty - eight women. Another group not on the time of labor onset were twenty - five women. The concentrations of PGE2 in maternal blood and amnlotic fluid were detected by radioimmune assay. Expression of bcl - 2 proteins in placental trophoblastic cells was detected by imnmnohistochemical assay. Results： （ 1 ） The concentrations of PGE2 in maternal blood and amniotic fluid in labor onset group were higher than not labor onset （ P 〈 0.05）. （2）Expression of bcl - 2 proteins in group labor onset was significantly lower than the group not labor onset（ P 〈 0.05）. （3） The expression of bcl - 2 proteins had a negative correlation with the concentrations of PG2 in maternal blood and amniotic fluid. Conclusion：It was considered that PGE2 and the apoptosis of placental trophoblastic cells induced by bcl - 2 may play important roles in the initiation of labor onset. PGE2 may influence the expression of bcl - 2.
出处
《中国优生与遗传杂志》
2005年第8期29-31,共3页
Chinese Journal of Birth Health & Heredity
