摘要
背景与目的整合素连接激酶(ILK)是一种丝/苏氨酸蛋白激酶,研究证明它是多种致瘤相关因素的上游交叉点,与肿瘤的形成、增殖、侵袭和转移密切相关。本文旨在探讨ILK在非小细胞肺癌(NSCLC)组织中的表达及其与组织类型、肿瘤分化、分期、淋巴结转移及预后的关系,并分析其与表皮钙粘素(E-cadherin)是否存在相关性。方法采用S-P免疫组化方法和WesternBlot法,检测NSCLC组织及相应癌旁肺组织中ILK和E-cadherin的表达情况,并结合临床和病理资料进行分析。结果免疫组化结果显示:ILK在69.7%(53/76)的NSCLC组织中阳性表达,其中鳞癌阳性率75.0%(33/44),腺癌阳性率62.5%(20/32),其表达与组织类型无关(P=0.247),与肺癌分期(P=0.002)、淋巴结转移(P=0.006)显著正相关,与肺癌分化程度(P=0.009)、患者生存时间(P=0.006)显著负相关。ILK与E-cadherin间的表达呈显著负相关(P<0.001)。WesternBlot结果显示ILK在肺癌组织中的表达显著高于癌旁正常肺组织(P=0.0002),其表达与肺癌的分化(P=0.0001)和E-cadherin的表达(P=0.006)显著负相关。结论在NSCLC中,ILK是肿瘤相关信号通路中的交叉位点,可与许多致瘤因子相互作用,从而促进肿瘤的发生、发展、侵袭和转移。ILK与E-cadherin间显著负相关,其对E-cadherin的影响可能是ILK发挥作用的机制之一。ILK可作为判断NSCLC患者预后的参考指标,ILK和E-cadherin的联合检测将有助于更好地判断肺癌患者的预后情况。
Background and objective Integrin-linked kinase （ILK） is a Ser/Thr protein kinase. Many studies have showed that ILK was closely related to occurrence, proliferation, invasion and metastasis in many malignant tumors, and it appeared to be an upstream cross point of tumor-associated factors. The aim of this study is to explore the relationship between the expression of ILK and some clinical pathological factors in human non-small cell lung cancer （NSCLC） , and analyze whether there is relativity between ILK and E-cadherin. Methods Immunohistochemical S-P method was adopted to detect the expression of ILK and E-cadherin proteins in 76 NSCLC cases with the neighboring noncancerous tissue, and the expressions of them in 30 fresh NSCLC samples were determined with Western Blot assay. Results Immunohistochemically, the overexpression of ILK protein in NSCLC was 53/76 （69.7 % ）, including 33/44 （75.0 % ） squamous cell carcinoma and 20/32 （62.5 % ） adenocarcinoma, but its expression was not related to the histological type （P=0. 247）. Ex pressionof ILK was related to differentiation （rs =-0.296, P=0.009）, lymph node metastasis （rs=0.311, P=0. 006） and clinical stage （rs=0. 350, P=0. 002）. Moreover, Kaplan Meier survival estimates showed a significant correlation between ILK expression and patient survival in Log rank test （P=0. 006）. Overexpres- sion of ILK in NSCLC was associated with unfavorable prognosis. An inverse correlation between the levels of ILK and E-cadherin was found （rs= 0. 514, P〈0. 001）. Western Blot result showed that the level of ILK in the tumor tissues was noticeably higher than that in the normal tissues （t=-6. 811, P=0. 0002）, and an inverse correlation between the levels of ILK and E-cadherin was proved （P= 0. 001）. Conclusion In NSCLC, ILK can interact with some tumor associated factors, through which it appears to be involved in several oncogenesis-related events, including promotion of cell survival, as well as cell migration and invasion. I
出处
《中国肺癌杂志》
CAS
2005年第4期291-296,共6页
Chinese Journal of Lung Cancer
关键词
肺肿瘤
癌
非小细胞肺
整合素连接激酶
表皮钙粘素
Lung neoplasms Carcinoma Non-small cell lung Integrin-linked kinase Ecadherin
