摘要
目的探讨硫酸脱氢表雄酮(DHEAS)对胰岛β细胞株MIN6葡萄糖刺激的胰岛素分泌的影响。方法以葡萄糖刺激浓度为2.8mmol/L和16.7mmol/L的对数生长期MIN6细胞作为实验对象,分别以1、5、10μmol/LDHEAS干预10min和24h(不同浓度DHEAS组),以5μmol/LDHEAS或与10μmol/L糖皮质激素受体阻断剂RU486联合干预24h(DHEAS和DHEAS+RU486组),设立空白对照组。ELISA法测定细胞培养上清液中胰岛素分泌量,Real-TimePCR检测细胞胰岛素mRNA表达。结果干预后10min和24h时点,5μmol/L和10μmol/LDHEAS组MIN6细胞培养上清液中的胰岛素分泌量均显著高于空白对照组(P<0.05);干预后24h时点,DHEAS+RU486组与DHEAS组MIN6细胞培养上清液中胰岛素分泌量比较差异无统计学意义(P>0.05),但均显著高于空白对照组(P<0.05)。干预后24h时点,5μmol/L和10μmol/LDHEAS组MIN6细胞胰岛素mRNA表达均显著高于空白对照组(P<0.05)。结论 DHEAS对MIN6细胞葡萄糖刺激的胰岛素分泌具有促进作用,且其核内信号通路可能不经糖皮质激素受体介导。
Objective To investigate the effects of dehydroepiandrosterone-sulfate(DHEAS) on glucose-stimulated insulin secretion in pancreatic islets β cell lines(MIN6).Methods MIN6 cells at exponential phase of growth stimulated by glucose(2.8 mmol/L and 16.7 mmol/L) were served as study objectives.Cells were treated by DHEAS(1,5 and 10 μmol/L) for 10 min and 24 h(1 μmol/L DHEAS group,5 μmol/L DHEAS group and 10 μmol/L DHEAS group),and DHEAS group(treated by 5 μmol/L DHEAS for 24 h) and DHEAS+RU486 group(treated by 5...
出处
《上海交通大学学报（医学版）》
CAS
CSCD
北大核心
2011年第1期1-4,共4页
Journal of Shanghai Jiao tong University:Medical Science
基金
国家自然科学基金(30570882)~~
关键词
硫酸脱氢表雄酮
胰岛Β细胞
葡萄糖刺激胰岛素分泌
dehydroepiandrosterone-sulfate
pancreatic β cells
glucose-stimulated insulin secretion
