摘要
目的研究犬脂肪干细胞(AdiposeDividedStromalCells,ADSCs)作为种子细复合珊瑚材料体外培养的生长特性。方法分离犬ADSCs,成骨诱导并转染绿色荧光蛋白(GFP)后,复合珊瑚材料培养。检测细胞总数,激光共聚焦观察细胞形态。结果ADSCs-珊瑚复合物体外增殖良好。激光共聚焦显示培养2周后细胞呈老化形态。结论GFP转染不影响细胞传代、增殖。细胞-材料复合物体外培养7天后回植较为适宜。
Objective To explore the proper in vitro co-culture time of ADSCs-coral construct before transplant. Methods ADSCs isolated were GFP labeled by RV-GFP transfection and cultured in osteogenic-induced. The construct were examined from total cells number, and imaged by laser confocal scanning microscope. Results ADSCs proliferated well in coral scaffords .The construct cultured for more than 2 weeks were effete,observed by laser confocal. Conclusion The GFP label has no negative effect in ADSCs passage and proliferation. The constructs cultured for 7 days is proper to transplant.
出处
《组织工程与重建外科杂志》
2006年第6期316-317,329,共3页
Journal of Tissue Engineering and Reconstructive Surgery
