摘要
[目的]探讨MSH2和MSH6蛋白在衰老SD大鼠脏器组织中的表达及其意义.[方法]用Western Blot方法检测8,52,104周SD大鼠脑、肺脏、结肠、小肠、肝脏、心脏、肌肉、肾脏、胃、胰腺、脾脏和前列腺等12个脏器组织中错配修复蛋白MSH2和MSH6的表达水平.[结果]MSH2蛋白在脑、肺脏、结肠、心脏、肌肉、脾脏、胃和前列腺等大部分脏器组织中的表达水平随着年龄的增长而逐渐下降;而MSH6蛋白仅在肺脏、小肠和前列腺等脏器组织中随着年龄的增长而逐渐下降.[结论]错配修复蛋白MSH2与细胞衰老有密切关联.
OBJECTIVE To study the expression and its significance of MSH2 and MSH6 proteins in tissues of aging SD rats.METHODSThe expression of mismatch repair protein MSH2 and MSH6 were detected by Western Blot in tissues of brain,lung,large intestine,small intestine,liver,heart,muscle,kidney,stomach,pancreas,spleen and prostate of 8,52 and 104 weeks old of aging SD rats,respectively.RESULTSThe expression of MSH2 in brain,lung,large intestine,heart,muscle,stomach,pancreas,spleen and prostate were decreased along with increase of age,and the expression of MSH6 only in lung,small intestine and prostate were decreased along with increase of age.CONCLUSIONThe expression of mismatch repair protein MSH2 is closely correlated with the cell senescence.
出处
《延边大学医学学报》
CAS
2010年第4期249-250,共2页
Journal of Medical Science Yanbian University
关键词
衰老
错配修复蛋白
大鼠
aging
mismatch repair protein
rats
