摘要
目的:探讨聚乳酸-壳聚糖-明胶(PLA-Chitosan-Gelatine)生物复合支架在牙周组织工程应用中的可行性。方法:HE染色和扫描电镜(SEM)观察支架微观结构。四甲基偶氮哇盐(MTT)比色分析法检测支架浸提液(SLL)细胞毒性。实验分为实验组及对照组;在490 nm波长下用酶标仪测定每孔的吸光度值(OD),计算细胞相对增殖率(RGR),根据RGR值进行支架细胞毒性分级。结果:支架纵切面呈多孔网状结构,孔间相互交连,其孔径介于20～200μm之间,孔径由光滑面向粗糙面递增。实验组中除100%SLL组RGR为96%外,其余各实验组RGR均大于100%。各实验组OD均值与阴性对照组OD均值差异无统计学意义(P>0.05),实验组和阴性对照组OD值均与阳性对照组OD均值有统计学差异(P<0.05)。结论:PLA-Chitosan-Gelatine支架三维立体递变性孔径和多孔结构能为成纤维细胞提供良好的培养环境,且无细胞毒性,符合生物学材料要求。
Objective: To observe the scaffold's structure of the PLA-Chitosan-Gelatin with pore size gradient,determine its cytotoxicity level,and investigate the possibility of the bio-composite scaffold in periodontal tissue engineering.Methods: The scaffold's structure were observed by HE stain and scanning electron microscopy.The cytotocity of the scaffold leaching liquor was tested by MTT assay.The experiment consisted of the experimental groups and the control group;The optical density of each well was measured at 490 nm wavelength using control reader and cell relative growth rates(RGR) were calculated.The cytotoxicity of the scaffold was graded according to RGR.Results: The scaffold showed reticular porous structure,inter-cross-linked pore,between 20 to 200 micron gradient pore size,increasing pore size by degrees from the smooth surface to the rough surface by SEM.The favourable hole structure existed in the culture medium after two weeks by HE stain.The 100% SLL group's RGR was 96%,the rest of the experimental group's RGR were greater than 100%.There was no significant difference between the experimental groups OD values and the negative control groups OD values(P> 0.05).Significant difference existed between the experimental groups and the positive control group,and between the negative control group and the positive control group on OD values(P<0.05).Conclusion: The PLA-Chitosan-Gelatine scaffold's three-dimensional,pore size and gradient provides a good training environment for fibroblasts,which has no cytotoxic,and it is in line with the requirements of biological materials.
出处
《天津医科大学学报》
2010年第4期594-597,共4页
Journal of Tianjin Medical University
关键词
聚乳酸
壳聚糖
明胶
支架
细胞毒性
牙周组织工程
Polylactic acid
Chitosan
Gelatine
Scaffold
Cytotoxicity
Periodontal tissue engineering
