摘要
目的:研究松果体和褪黑激素(Mel)是否通过下丘脑影响腹腔巨噬细胞功能.方法:松果体切除术;腹腔巨噬细胞化学发光测定;下丘脑地诺前列酮放射免疫测定;下丘脑注射Mel.结果:松果体切除后腹腔巨噬细胞化学发光值降低,下丘脑地诺前列酮含量升高,16:00 ip Mel(10 μg kg^(-1)d^(-1)×7d)可使其恢复,并升高正常大鼠腹腔巨噬细胞化学发光值,降低其下丘脑地诺前列酮含量.腹腔巨噬细胞化学发光值与下丘脑地诺前列酮含量的变化存在负相关(相关系数,r=-0.78,P<0.01).于下丘脑注射Mel 2μg,能提高正常大鼠和松果体切除大鼠腹腔巨噬细胞化学发光值.结论:下丘脑是松果体Mel影响腹腔巨噬细胞功能的主要作用部位之一.
AIM:To study whether the hypothalamus was a major relay for the action of melatonin (Mel) on peritoneal macrophage (PM ) function. METHODS:Pinealectomy, luminol-dependent chemiluminescence (CD, hypothalamic dinoprostone radioimmuno-assay, and intrahypothalamic injection of Mel were done in rats kept under light:dark 12 h : 12 h. RESULTS: CL value of PM was decreased and hypothalamic dinoprostone content was elevated after pinealectomy, which were restored by Mel (10μg kg-1 d-1 ip at 16:00 for 7 d). The same treatment of Mel increased CL value of PM 0 and depressed hypothalamic dinoprostone production in intact rats. CL value of PM 0 showed negative relation to hypothalamic dinoprostone with r=-0.78 (P<0.01). Intrahypothalamic injection of Mel (2μg) enhanced CL value of PM 0 in normal and pinealectomized rats. CONCLUSION: The hypothalamus is a main site of pineal Mel action upon PM 0 function.
出处
《中国药理学报》
CSCD
1995年第5期435-437,共3页
Acta Pharmacologica Sinica
关键词
松果林
褪黑激素
腹腔巨噬细胞
化学发光
下丘脑
pineal body
melatonin
peritoneal macrophages
chemiluminescence
hypothalamus
dinoprostone
