摘要
建立了高效液相色谱测定维拉帕米血药浓度的新方法。血浆样品用正己烷－乙醚（１：１，Ｖ／Ｖ）提取，以ＺｏｒｂａｘＳｉｌｉｃａ为固定相，０.０５ｍｏｌ／Ｌ磷酸二氢铵－乙腈－甲醇－三乙胺（５０：２５：２５：０.２５，Ｖ／Ｖ）为流动相，紫外２３２ｎｍ处测定。采用外标法峰高定量。血药浓度在２.５～４００，ｎｇ／ｍｌ线性关系良好，ｒ＝０.９９９６。最低检测浓度为２.０ｎｇ／ｍｌ。回收率达９５.１１％～１０１.９９％，日内、日间ＲＳＤ分别为３.７６％～６.４５％和６.０６％～７.４６％。
A HPLC method was developed for the determination of verapamil in human plasma.Verapamil was extracted from plasma using mixture of ether and n-hexane(1:1).A Zorbax Silica column(250 mm×4. 6 mm ID,5μm)was used and a UV detector was set at 232 nm,The mobile phase consisted of 0. 05 mol/L NH4H2PO4,acetonitrile,methanol and triethylamine at ratio of 50:25:25:0.25(adjusted to pH6.0 with phosphoric acid).There was no interference with its metabolites and endogenous components in plasma.The standard curve was linear over the concentraton range of 2.5～400.0 ng/ml(r=0.9996).The recoveries were 95.11%～101.99% and the minimum detection concentration of verapamil in plasma was 2 ng/ml.Analysis of plasma samples collected from healthy subjects demonstrated that this assay was applicable to clinical and pharmacokinetic studies.
出处
《中国药科大学学报》
CAS
CSCD
北大核心
1995年第6期347-349,共3页
Journal of China Pharmaceutical University
